Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy

Prihantono Prihantono; Daniel Sampepajung; Andi Asadul Islam; Mochammad Hatta; Warsinggih Rahardjo; Christian Binekada; Eddy Herman Tanggo

doi:doi:10.11648/j.ajcem.20170501.13

Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy

Prihantono Prihantono, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta, Warsinggih Rahardjo, Christian Binekada, Eddy Herman Tanggo

Published in American Journal of Clinical and Experimental Medicine (Volume 5, Issue 1)

Received: 7 December 2016 Accepted: 20 December 2016 Published: 19 January 2017

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Abstract

Background: Proliferation is a distinct hallmarks of cancer. Ki-67 designated as a marker of proliferation in solid tumors. The proliferative activity of tumor demonstrated by expression of Ki-67 in breast cancer has been associated with a poor prognosis. Changes in the relative proportions of Ki-67 can be observed during chemotherapy and may correlated with clinical response in breast cancer. Purpose: Evaluate changes in mRNA expression of proliferation marker Ki-67 in breast cancer patients pre- and post-chemotherapy in relation with clinical response to chemotherapy. Method: This is a longitudinal study, 30 subjects breast cancer tissue samples pre- and post-chemotherapy using cyclophosphamide, adriamycin, 5FU regiment. We using qRT-PCR techniques to detect mRNA expression of Ki-67. Chemotherapy response is calculated using RECIST criteria. Results: Mean value of Ki-67 mRNA expression on breast cancer patients pre-chemotheraphy was 11.837±0.360. Mean value of Ki-67 mRNA expression on breast cancer patients post-chemotheraphy was 11.241±1.971. There was no significant correlation between expression of Ki-67 mRNA prechemotherapy with clinical response to chemotherapy, p = 0.862 (p ≥0.05). There is a positive correlation between velocity of Ki-67 mRNA expression with clinical response with value of r = 0.378, this correlation was significant with p = 0.020 (p<0.05). Conclusion: Chemotherapy cause decrease in mRNA expression of Ki-67. There is insignificant correlation between expression of mRNA Ki-67 baseline with chemotherapy response. Velocity of Ki-67 mRNA expression has significant correlation with clinical response to chemotherapy.

Published in	American Journal of Clinical and Experimental Medicine (Volume 5, Issue 1)
DOI	10.11648/j.ajcem.20170501.13
Page(s)	10-14
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Breast Cancer, Chemotherapy, mRNA, Ki-67, QRT-PCR

References

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APA Style

Prihantono Prihantono, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta, Warsinggih Rahardjo, et al. (2017). Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy. American Journal of Clinical and Experimental Medicine, 5(1), 10-14. https://doi.org/10.11648/j.ajcem.20170501.13

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ACS Style

Prihantono Prihantono; Daniel Sampepajung; Andi Asadul Islam; Mochammad Hatta; Warsinggih Rahardjo, et al. Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy. Am. J. Clin. Exp. Med. 2017, 5(1), 10-14. doi: 10.11648/j.ajcem.20170501.13

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AMA Style

Prihantono Prihantono, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta, Warsinggih Rahardjo, et al. Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy. Am J Clin Exp Med. 2017;5(1):10-14. doi: 10.11648/j.ajcem.20170501.13

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@article{10.11648/j.ajcem.20170501.13,
  author = {Prihantono Prihantono and Daniel Sampepajung and Andi Asadul Islam and Mochammad Hatta and Warsinggih Rahardjo and Christian Binekada and Eddy Herman Tanggo},
  title = {Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {1},
  pages = {10-14},
  doi = {10.11648/j.ajcem.20170501.13},
  url = {https://doi.org/10.11648/j.ajcem.20170501.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170501.13},
  abstract = {Background: Proliferation is a distinct hallmarks of cancer. Ki-67 designated as a marker of proliferation in solid tumors. The proliferative activity of tumor demonstrated by expression of Ki-67 in breast cancer has been associated with a poor prognosis. Changes in the relative proportions of Ki-67 can be observed during chemotherapy and may correlated with clinical response in breast cancer. Purpose: Evaluate changes in mRNA expression of proliferation marker Ki-67 in breast cancer patients pre- and post-chemotherapy in relation with clinical response to chemotherapy. Method: This is a longitudinal study, 30 subjects breast cancer tissue samples pre- and post-chemotherapy using cyclophosphamide, adriamycin, 5FU regiment. We using qRT-PCR techniques to detect mRNA expression of Ki-67. Chemotherapy response is calculated using RECIST criteria. Results: Mean value of Ki-67 mRNA expression on breast cancer patients pre-chemotheraphy was 11.837±0.360. Mean value of Ki-67 mRNA expression on breast cancer patients post-chemotheraphy was 11.241±1.971. There was no significant correlation between expression of Ki-67 mRNA prechemotherapy with clinical response to chemotherapy, p = 0.862 (p ≥0.05). There is a positive correlation between velocity of Ki-67 mRNA expression with clinical response with value of r = 0.378, this correlation was significant with p = 0.020 (p<0.05). Conclusion: Chemotherapy cause decrease in mRNA expression of Ki-67. There is insignificant correlation between expression of mRNA Ki-67 baseline with chemotherapy response. Velocity of Ki-67 mRNA expression has significant correlation with clinical response to chemotherapy.},
 year = {2017}
}

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TY - JOUR
T1 - Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy
AU - Prihantono Prihantono
AU - Daniel Sampepajung
AU - Andi Asadul Islam
AU - Mochammad Hatta
AU - Warsinggih Rahardjo
AU - Christian Binekada
AU - Eddy Herman Tanggo
Y1 - 2017/01/19
PY - 2017
N1 - https://doi.org/10.11648/j.ajcem.20170501.13
DO - 10.11648/j.ajcem.20170501.13
T2 - American Journal of Clinical and Experimental Medicine
JF - American Journal of Clinical and Experimental Medicine
JO - American Journal of Clinical and Experimental Medicine
SP - 10
EP - 14
PB - Science Publishing Group
SN - 2330-8133
UR - https://doi.org/10.11648/j.ajcem.20170501.13
AB - Background: Proliferation is a distinct hallmarks of cancer. Ki-67 designated as a marker of proliferation in solid tumors. The proliferative activity of tumor demonstrated by expression of Ki-67 in breast cancer has been associated with a poor prognosis. Changes in the relative proportions of Ki-67 can be observed during chemotherapy and may correlated with clinical response in breast cancer. Purpose: Evaluate changes in mRNA expression of proliferation marker Ki-67 in breast cancer patients pre- and post-chemotherapy in relation with clinical response to chemotherapy. Method: This is a longitudinal study, 30 subjects breast cancer tissue samples pre- and post-chemotherapy using cyclophosphamide, adriamycin, 5FU regiment. We using qRT-PCR techniques to detect mRNA expression of Ki-67. Chemotherapy response is calculated using RECIST criteria. Results: Mean value of Ki-67 mRNA expression on breast cancer patients pre-chemotheraphy was 11.837±0.360. Mean value of Ki-67 mRNA expression on breast cancer patients post-chemotheraphy was 11.241±1.971. There was no significant correlation between expression of Ki-67 mRNA prechemotherapy with clinical response to chemotherapy, p = 0.862 (p ≥0.05). There is a positive correlation between velocity of Ki-67 mRNA expression with clinical response with value of r = 0.378, this correlation was significant with p = 0.020 (p<0.05). Conclusion: Chemotherapy cause decrease in mRNA expression of Ki-67. There is insignificant correlation between expression of mRNA Ki-67 baseline with chemotherapy response. Velocity of Ki-67 mRNA expression has significant correlation with clinical response to chemotherapy.
VL - 5
IS - 1
ER -

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Author Information

Prihantono Prihantono

Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Daniel Sampepajung

Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Andi Asadul Islam

Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Mochammad Hatta

Molecular Biology and Immunology Laboratory, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Warsinggih Rahardjo

Department of Surgery, Faculty of Medicine, Hasanuddin University, Makassar, Indonesia
Christian Binekada

Department of Surgery, Faculty of Medicine, Haluoleo University, Kendari, Indonesia
Eddy Herman Tanggo

Department of Surgery, Faculty of Medicine, Airlangga University, Surabaya, Indonesia

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APA Style

Prihantono Prihantono, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta, Warsinggih Rahardjo, et al. (2017). Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy. American Journal of Clinical and Experimental Medicine, 5(1), 10-14. https://doi.org/10.11648/j.ajcem.20170501.13

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ACS Style

Prihantono Prihantono; Daniel Sampepajung; Andi Asadul Islam; Mochammad Hatta; Warsinggih Rahardjo, et al. Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy. Am. J. Clin. Exp. Med. 2017, 5(1), 10-14. doi: 10.11648/j.ajcem.20170501.13

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AMA Style

Prihantono Prihantono, Daniel Sampepajung, Andi Asadul Islam, Mochammad Hatta, Warsinggih Rahardjo, et al. Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy. Am J Clin Exp Med. 2017;5(1):10-14. doi: 10.11648/j.ajcem.20170501.13

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@article{10.11648/j.ajcem.20170501.13,
  author = {Prihantono Prihantono and Daniel Sampepajung and Andi Asadul Islam and Mochammad Hatta and Warsinggih Rahardjo and Christian Binekada and Eddy Herman Tanggo},
  title = {Profile of mRNA Expression of Ki-67 in Breast Cancer Patients Pre- and Post- Chemotherapy},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {5},
  number = {1},
  pages = {10-14},
  doi = {10.11648/j.ajcem.20170501.13},
  url = {https://doi.org/10.11648/j.ajcem.20170501.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20170501.13},
  abstract = {Background: Proliferation is a distinct hallmarks of cancer. Ki-67 designated as a marker of proliferation in solid tumors. The proliferative activity of tumor demonstrated by expression of Ki-67 in breast cancer has been associated with a poor prognosis. Changes in the relative proportions of Ki-67 can be observed during chemotherapy and may correlated with clinical response in breast cancer. Purpose: Evaluate changes in mRNA expression of proliferation marker Ki-67 in breast cancer patients pre- and post-chemotherapy in relation with clinical response to chemotherapy. Method: This is a longitudinal study, 30 subjects breast cancer tissue samples pre- and post-chemotherapy using cyclophosphamide, adriamycin, 5FU regiment. We using qRT-PCR techniques to detect mRNA expression of Ki-67. Chemotherapy response is calculated using RECIST criteria. Results: Mean value of Ki-67 mRNA expression on breast cancer patients pre-chemotheraphy was 11.837±0.360. Mean value of Ki-67 mRNA expression on breast cancer patients post-chemotheraphy was 11.241±1.971. There was no significant correlation between expression of Ki-67 mRNA prechemotherapy with clinical response to chemotherapy, p = 0.862 (p ≥0.05). There is a positive correlation between velocity of Ki-67 mRNA expression with clinical response with value of r = 0.378, this correlation was significant with p = 0.020 (p<0.05). Conclusion: Chemotherapy cause decrease in mRNA expression of Ki-67. There is insignificant correlation between expression of mRNA Ki-67 baseline with chemotherapy response. Velocity of Ki-67 mRNA expression has significant correlation with clinical response to chemotherapy.},
 year = {2017}
}

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