Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer

Na Yi; Lengge Si; Yuehong Wang; Lidao Bao

doi:doi:10.11648/j.ajcem.20150306.20

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Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer

Na Yi, Lengge Si, Yuehong Wang, Lidao Bao

Published in American Journal of Clinical and Experimental Medicine (Volume 3, Issue 6)

Received: 24 December 2015 Published: 30 December 2015

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Abstract

To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The mice were killed on the next day of administration discontinuance, and subcutaneous tumor tissues were collected. Quantitative fluorescence RT-PCR was used to detect the expression of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of H29 colon cancer mice. Apigenin raised the expression level of TGF-β1R II in H29 colon cancer tissues, which showed the most obvious effect in the middle-dose group, with a significant difference compared with the normal saline group (P<0.01). The Apigenin group of each dose could significantly lower the NF-κB expression level in H29 colon solid tumors, showing significant differences compared with the normal saline group (P<0.01). The middle-dose and high-dose Apigenin groups could significantly reduce the level of VEGF expression in tumor tissues of ICR mice with H29 colon cancer, and the high-dose group had most obvious effect, and there were significant difference among the middle-dose group, high-dose group and the normal saline group (P<0.01). The mechanism of anti-tumor effect of Apigenin might be the reason that Apigenin can raise the expression level of TGF-β1R II by down-regulating the expression of NF–κB and VEGF in tumor tissues of tumor-bearing mice, thereby inhibiting tumor angiogenesis and tumor cell proliferation, so as to achieve the anti-tumor effect.

Published in	American Journal of Clinical and Experimental Medicine (Volume 3, Issue 6)
DOI	10.11648/j.ajcem.20150306.20
Page(s)	378-382
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Apigenin, H29 Colon Cancer, TGF-β1RII, NF-κB, VEGF

References

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APA Style

Na Yi, Lengge Si, Yuehong Wang, Lidao Bao. (2015). Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer. American Journal of Clinical and Experimental Medicine, 3(6), 378-382. https://doi.org/10.11648/j.ajcem.20150306.20

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ACS Style

Na Yi; Lengge Si; Yuehong Wang; Lidao Bao. Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer. Am. J. Clin. Exp. Med. 2015, 3(6), 378-382. doi: 10.11648/j.ajcem.20150306.20

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AMA Style

Na Yi, Lengge Si, Yuehong Wang, Lidao Bao. Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer. Am J Clin Exp Med. 2015;3(6):378-382. doi: 10.11648/j.ajcem.20150306.20

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@article{10.11648/j.ajcem.20150306.20,
  author = {Na Yi and Lengge Si and Yuehong Wang and Lidao Bao},
  title = {Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {6},
  pages = {378-382},
  doi = {10.11648/j.ajcem.20150306.20},
  url = {https://doi.org/10.11648/j.ajcem.20150306.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150306.20},
  abstract = {To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The mice were killed on the next day of administration discontinuance, and subcutaneous tumor tissues were collected. Quantitative fluorescence RT-PCR was used to detect the expression of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of H29 colon cancer mice. Apigenin raised the expression level of TGF-β1R II in H29 colon cancer tissues, which showed the most obvious effect in the middle-dose group, with a significant difference compared with the normal saline group (P<0.01). The Apigenin group of each dose could significantly lower the NF-κB expression level in H29 colon solid tumors, showing significant differences compared with the normal saline group (P<0.01). The middle-dose and high-dose Apigenin groups could significantly reduce the level of VEGF expression in tumor tissues of ICR mice with H29 colon cancer, and the high-dose group had most obvious effect, and there were significant difference among the middle-dose group, high-dose group and the normal saline group (P<0.01). The mechanism of anti-tumor effect of Apigenin might be the reason that Apigenin can raise the expression level of TGF-β1R II by down-regulating the expression of NF–κB and VEGF in tumor tissues of tumor-bearing mice, thereby inhibiting tumor angiogenesis and tumor cell proliferation, so as to achieve the anti-tumor effect.},
 year = {2015}
}

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TY  - JOUR
T1  - Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer
AU  - Na Yi
AU  - Lengge Si
AU  - Yuehong Wang
AU  - Lidao Bao
Y1  - 2015/12/30
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150306.20
DO  - 10.11648/j.ajcem.20150306.20
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 378
EP  - 382
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150306.20
AB  - To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The mice were killed on the next day of administration discontinuance, and subcutaneous tumor tissues were collected. Quantitative fluorescence RT-PCR was used to detect the expression of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of H29 colon cancer mice. Apigenin raised the expression level of TGF-β1R II in H29 colon cancer tissues, which showed the most obvious effect in the middle-dose group, with a significant difference compared with the normal saline group (P<0.01). The Apigenin group of each dose could significantly lower the NF-κB expression level in H29 colon solid tumors, showing significant differences compared with the normal saline group (P<0.01). The middle-dose and high-dose Apigenin groups could significantly reduce the level of VEGF expression in tumor tissues of ICR mice with H29 colon cancer, and the high-dose group had most obvious effect, and there were significant difference among the middle-dose group, high-dose group and the normal saline group (P<0.01). The mechanism of anti-tumor effect of Apigenin might be the reason that Apigenin can raise the expression level of TGF-β1R II by down-regulating the expression of NF–κB and VEGF in tumor tissues of tumor-bearing mice, thereby inhibiting tumor angiogenesis and tumor cell proliferation, so as to achieve the anti-tumor effect.
VL  - 3
IS  - 6
ER  -

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Author Information

Na Yi

College of Pharmacy, Inner Mongolia Medical University, Hohhot, China
Lengge Si

College of Mongolian Medicine, Inner Mongolia Medical University, Hohhot, China
Yuehong Wang

College of Mongolian Medicine, Inner Mongolia Medical University, Hohhot, China
Lidao Bao

College of Pharmacy, Inner Mongolia Medical University, Hohhot, China

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APA Style

Na Yi, Lengge Si, Yuehong Wang, Lidao Bao. (2015). Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer. American Journal of Clinical and Experimental Medicine, 3(6), 378-382. https://doi.org/10.11648/j.ajcem.20150306.20

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ACS Style

Na Yi; Lengge Si; Yuehong Wang; Lidao Bao. Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer. Am. J. Clin. Exp. Med. 2015, 3(6), 378-382. doi: 10.11648/j.ajcem.20150306.20

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AMA Style

Na Yi, Lengge Si, Yuehong Wang, Lidao Bao. Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer. Am J Clin Exp Med. 2015;3(6):378-382. doi: 10.11648/j.ajcem.20150306.20

Copy | Download

@article{10.11648/j.ajcem.20150306.20,
  author = {Na Yi and Lengge Si and Yuehong Wang and Lidao Bao},
  title = {Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer},
  journal = {American Journal of Clinical and Experimental Medicine},
  volume = {3},
  number = {6},
  pages = {378-382},
  doi = {10.11648/j.ajcem.20150306.20},
  url = {https://doi.org/10.11648/j.ajcem.20150306.20},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajcem.20150306.20},
  abstract = {To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The mice were killed on the next day of administration discontinuance, and subcutaneous tumor tissues were collected. Quantitative fluorescence RT-PCR was used to detect the expression of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of H29 colon cancer mice. Apigenin raised the expression level of TGF-β1R II in H29 colon cancer tissues, which showed the most obvious effect in the middle-dose group, with a significant difference compared with the normal saline group (P<0.01). The Apigenin group of each dose could significantly lower the NF-κB expression level in H29 colon solid tumors, showing significant differences compared with the normal saline group (P<0.01). The middle-dose and high-dose Apigenin groups could significantly reduce the level of VEGF expression in tumor tissues of ICR mice with H29 colon cancer, and the high-dose group had most obvious effect, and there were significant difference among the middle-dose group, high-dose group and the normal saline group (P<0.01). The mechanism of anti-tumor effect of Apigenin might be the reason that Apigenin can raise the expression level of TGF-β1R II by down-regulating the expression of NF–κB and VEGF in tumor tissues of tumor-bearing mice, thereby inhibiting tumor angiogenesis and tumor cell proliferation, so as to achieve the anti-tumor effect.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Effects of Apigenin on the Expressions of TGF-β1R II, NF-κB and VEGF Genes in Tumor Tissues of Mice with H29 Colon Cancer
AU  - Na Yi
AU  - Lengge Si
AU  - Yuehong Wang
AU  - Lidao Bao
Y1  - 2015/12/30
PY  - 2015
N1  - https://doi.org/10.11648/j.ajcem.20150306.20
DO  - 10.11648/j.ajcem.20150306.20
T2  - American Journal of Clinical and Experimental Medicine
JF  - American Journal of Clinical and Experimental Medicine
JO  - American Journal of Clinical and Experimental Medicine
SP  - 378
EP  - 382
PB  - Science Publishing Group
SN  - 2330-8133
UR  - https://doi.org/10.11648/j.ajcem.20150306.20
AB  - To observe the effects of Apigenin on the expressions of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of mice with H29 colon cancer. Fifty ICR mice with H29 colon cancer were randomly divided into five groups: normal saline group, low-dose Apigenin group, middle-dose Apigenin group and high-dose Apigenin group and cyclophosphamide group. The mice were killed on the next day of administration discontinuance, and subcutaneous tumor tissues were collected. Quantitative fluorescence RT-PCR was used to detect the expression of TGF-β1R II, NF-κB and VEGF genes in tumor tissues of H29 colon cancer mice. Apigenin raised the expression level of TGF-β1R II in H29 colon cancer tissues, which showed the most obvious effect in the middle-dose group, with a significant difference compared with the normal saline group (P<0.01). The Apigenin group of each dose could significantly lower the NF-κB expression level in H29 colon solid tumors, showing significant differences compared with the normal saline group (P<0.01). The middle-dose and high-dose Apigenin groups could significantly reduce the level of VEGF expression in tumor tissues of ICR mice with H29 colon cancer, and the high-dose group had most obvious effect, and there were significant difference among the middle-dose group, high-dose group and the normal saline group (P<0.01). The mechanism of anti-tumor effect of Apigenin might be the reason that Apigenin can raise the expression level of TGF-β1R II by down-regulating the expression of NF–κB and VEGF in tumor tissues of tumor-bearing mice, thereby inhibiting tumor angiogenesis and tumor cell proliferation, so as to achieve the anti-tumor effect.
VL  - 3
IS  - 6
ER  -

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