Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.
| Published in | American Journal of Agriculture and Forestry (Volume 9, Issue 3) |
| DOI | 10.11648/j.ajaf.20210903.17 |
| Page(s) | 141-146 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2021. Published by Science Publishing Group |
Camellia oleifera, Phosphate-solubilizing Bacteria, 16S rRNA, Phosphate Solubilization Index
| [1] | Khan, M. S., Zaidi, A., Ahemad, M., Oves, M., & Wani, P. A. 2010. Plant growth promotion by phosphate solubilizing fungi – current perspective. Arch. Agron. Soil Sci., 56, 73–98. |
| [2] | Vance, C., Uhde-Stone, C., & Allan, D. L. 2003. Phosphorus acquisition and use: critical adaptations by plants for securing a nonrenewable resource. New Phytol., 157, 423–447. |
| [3] | Tan, X. F., Ma, L. Y., Li, D. F., Yao, X. H., Pei, D., Su, S. C., Mao, Y. M., Li, J. A., & Yuan, D. Y. 2012. Industrialization development strategy of woody grain and oil in China. Nonwood For. Res., 30, 1–5. |
| [4] | Chen, Y. Z., Wang, D. B., & Su, Y. C. 2001. The fatty acid composition of Camellia oleifera. Nonwood For. Res., 14, 1–4. |
| [5] | Liao, W., Wen, R., Ma, H., Chen, G., Zhang, N., Wang, D., & Ye, H. 2014. Nutrient elements content in soil and leaf of adult Camellia oleifera forests main areas in central and northern Guangxi. Chinese Journal of Tropical Agriculture, 34, 18–21. |
| [6] | Liu, J., Wu, L., Chen, D., Li, M., & Wei, C. Soil quality assessment of different Camellia oleifera stands in mid-subtropical China. Appl. Soil Ecol, 2017, 113, 29–35. |
| [7] | Pikovskaya, R. I. 1948. Mobilization of phosphorus in soil in connection with vital activity of some microbial species. Microbiology, 17, 362–370. |
| [8] | Gyaneshwar, P., Kumar, G. N., Parekh, L. J., & Poole, P. S. 2002. Role of soil microorganisms in improving P nutrition of plants. Plant Soil, 245, 83–93. |
| [9] | Chen, Y. P., Rekha, P. D., Arun, A. B., Shen, F. T., Lai, W. A., & Young, C. C. 2006. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilizing abilities. Appl. Soil Ecol., 34, 33–41. |
| [10] | Rodriguez, H., & Fraga, R. 1999. Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnol. Adv., 17, 319–339. |
| [11] | Maria, R., Sumera, Y., Mahreen, Y., Claudia, B., Mika, T., & Thomas, R. 2021. The wheat growth-promoting traits of Ochrobactrum and Pantoea species, responsible for solubilization of different P sources, are ensured by genes encoding enzymes of multiple P-releasing pathways. Microbiol. Res., 246, 126703. |
| [12] | Sharma, S. B., Sayyed, R. Z., Trivedi, M. H., & Gobi, T. A. 2013. Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus, 2, 587. |
| [13] | Boubekri, K., Soumare, A., Mardad, I., Lyamlouli, K., Hafidi, M., Ouhdouch, Y., & Kouisni, L. 2021. The screening of potassium- and phosphate-solubilizing Actinobacteria and the assessment of their ability to promote wheat growth parameters. Microorganisms, 9, 470. |
| [14] | Wang, S., Zhang, L. P., Zhang, Y., Hao, F. F., Xiao, J. X., & Hu D. N. 2015. Screening, identification and phosphate solubilizing capability of phosphate solubilizing bacteria in rhizosphere of Camellia oleifera Abel at red soil region. Forest Res., 28, 409–416. |
| [15] | Liu, X., Fu, D., Chen, L., Yang, W., Li, D., & Fu, H. 2015. Isolation, identification and phosphate-solubilizing capacity of phosphate-solubilizing bacteria from the rhizosphere of Camellia. Biotechnology Bulletin, 31, 169–173. |
| [16] | Liu, X., Fu, D., Jia, C., & Chen, Q. 2016. Isolation, identification and culture condition of phosphate-solubilizing bacteria derived from Camellia rhizosphere soil. Southwest China Journal of Agricultural Sciences, 29, 2637–2642. |
| [17] | Chen, D. A., Wei, X. W., Zhang, M., Cheng, W., Wang, Y. S., Li, Y. L., Wu, SD., & Yi, H. W. 2020. Isolation, identification and phosphate solubilizing capacity of organophosphorus solubilizing bacteria in rhizosphere soil of Camellia oleifera. Agricultural Science & Technology, 21, 41–47. |
| [18] | Wu, F., Li, J., Chen, Y., Zhang, L., Zhang, Y., Wang, S., Shi, X., Li, L., & Liang, J. 2019. Effects of phosphate solubilizing bacteria on the growth, photosynthesis, and nutrient uptake of Camellia oleifera Abel. Forests, 10, 348. |
| [19] | Tang, Y., Lu, L., Yang, Q. Y., & Yu, G. H. 2006. Application and research progress of phosphate-solubilizing microorganisms. Tianjin Agricultural Sciences, 7, 1–5. |
| [20] | Sun, Q., Li, J., Finlay, R. D., & Lian, B. 2019. Oxalotrophic bacterial assemblages in the ectomycorrhizosphere of forest trees and their effects on oxalate degradation and carbon fixation potential. Chem. Geol., 514, 54–64. |
| [21] | Kumar, S., Stecher, G., & Tamura, K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol. Biol. Evol., 33, 1870–1874. |
| [22] | Edi Premono, M., Moawad, A. M., & Vlek, P. L. G. 1996. Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian J. Crop Sci., 11, 13–23. |
| [23] | R Core Team. 2020. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. URL https://www.R-project.org/. |
| [24] | Sengupta, A., Gunri, S. K., Biswas, T., & Saha, J. 2018. Efficacies of freshly isolated phosphate solubilising bacteria (PSB) on growth promotion in groundnut (Arachis hypogaea L.) upon commonly used PSB biofertilizers in eastern India. Legume Res., LR-4020, 1–7. |
| [25] | Yasmin, H., & Bano, A. 2011. Isolation and characterization of phosphate-solubilizing bacteria from rhizosphere soil of weeds of Khewra salt range and Attock. Pak. J. Bot., 43, 1663–1668. |
| [26] | Kucey, R. M. N., Janzen, H. H., & Legget, M. E. 1989. Microbial mediated increases in plant-available phosphorus. Adv. Agron., 42, 199–228. |
| [27] | Paul, D., & Sinha, S. N. 2017. Isolation and characterization of phosphate solubilizing bacterium Pseudomonas aeruginosa KUPSB12 with antibacterial potential from River Ganga, India. Annals of Agricultural Sciences, 15, 130. |
| [28] | Mohamed, E. A. H., Farag, A. G., & Youssef, S. A. 2018. Phosphate solubilization by Bacillus subtilis and Serratia marcescens isolated from tomato plant rhizosphere. J. Environ. Prot., 9, 266–277. |
| [29] | Zhou, J., Lu, M., Zhang, C., Qu, X., Liu, Y., Yang, J., & Yuan, J. 2020. Isolation and functional characterisation of the PHT1 gene encoding a high-affinity phosphate transporter in Camellia oleifera. J. Hortic. Sci. Biotech., 95, 553–564. |
| [30] | Hütsch, B. W., Augustin, J., Merbach, W. 2002. Plant rhizodeposition: an important source for carbon turnover in soils. J. Plant Nutr. Soil Sci., 165, 397407. |
| [31] | Wang, D. X., Zhang, N. Y., & Chen, G. C. 2011. Effects of AM fungi on the growth and drought-resistance of Camellia oleifera. Guangxi Forestry Science, 40, 259–273. |
| [32] | Smith, S. E., & Read, D. 2008. Mycorrhizal Symbiosis, 3 ed., Publisher: Elsevier Academic Press Inc, Netherlands. |
| [33] | Scheublin T. R., Sander, I. R., Keel, C. K., & van der Meer, J. R. 2010. Characterisation of microbial communities colonising the hyphal surfaces of arbuscular mycorrhizal fungi. ISME J., 4, 752–763. |
| [34] | Jiang, F., Zhang, L., Zhou J., George, T. S., & Feng, G. 2021. Arbuscular mycorrhizal fungi enhance mineralisation of organic phosphorus by carrying bacteria along their extraradical hyphae. New Phytol., 230, 304–315. |
| [35] | Emmett, B. D., Lévesque-Tremblay, V., Harrison, M. J. 2021. Conserved and reproducible bacterial communities associate with extraradical hyphae of arbuscular mycorrhizal fungi. ISME J. https://doi.org/10.1038/s41396-021-00920-2. |
| [36] | Venkateswarlu, B., Rao, A. V., & Raina, P. 1984. Evaluation of phosphorus solubilisation by microorganisms isolated from arid soil. Journal of the Indian Society of Soil Science, 32, 273–277. |
APA Style
Qibiao Sun, Yanfen Liu, Yan Tang, Peiyu Zhang, Yao Tong, et al. (2021). The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. American Journal of Agriculture and Forestry, 9(3), 141-146. https://doi.org/10.11648/j.ajaf.20210903.17
ACS Style
Qibiao Sun; Yanfen Liu; Yan Tang; Peiyu Zhang; Yao Tong, et al. The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. Am. J. Agric. For. 2021, 9(3), 141-146. doi: 10.11648/j.ajaf.20210903.17
AMA Style
Qibiao Sun, Yanfen Liu, Yan Tang, Peiyu Zhang, Yao Tong, et al. The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. Am J Agric For. 2021;9(3):141-146. doi: 10.11648/j.ajaf.20210903.17
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Download@article{10.11648/j.ajaf.20210903.17,
author = {Qibiao Sun and Yanfen Liu and Yan Tang and Peiyu Zhang and Yao Tong and Gang He and Xiaohong Ji and Zhenying He and Jianping Ouyang and Hongfang Zhang and Ye Chen},
title = {The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan},
journal = {American Journal of Agriculture and Forestry},
volume = {9},
number = {3},
pages = {141-146},
doi = {10.11648/j.ajaf.20210903.17},
url = {https://doi.org/10.11648/j.ajaf.20210903.17},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20210903.17},
abstract = {Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.},
year = {2021}
}
TY - JOUR T1 - The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan AU - Qibiao Sun AU - Yanfen Liu AU - Yan Tang AU - Peiyu Zhang AU - Yao Tong AU - Gang He AU - Xiaohong Ji AU - Zhenying He AU - Jianping Ouyang AU - Hongfang Zhang AU - Ye Chen Y1 - 2021/05/31 PY - 2021 N1 - https://doi.org/10.11648/j.ajaf.20210903.17 DO - 10.11648/j.ajaf.20210903.17 T2 - American Journal of Agriculture and Forestry JF - American Journal of Agriculture and Forestry JO - American Journal of Agriculture and Forestry SP - 141 EP - 146 PB - Science Publishing Group SN - 2330-8591 UR - https://doi.org/10.11648/j.ajaf.20210903.17 AB - Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P. VL - 9 IS - 3 ER -
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