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The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan

Received: 14 May 2021     Accepted: 26 May 2021     Published: 31 May 2021
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Abstract

Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.

Published in American Journal of Agriculture and Forestry (Volume 9, Issue 3)
DOI 10.11648/j.ajaf.20210903.17
Page(s) 141-146
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Camellia oleifera, Phosphate-solubilizing Bacteria, 16S rRNA, Phosphate Solubilization Index

References
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    Qibiao Sun, Yanfen Liu, Yan Tang, Peiyu Zhang, Yao Tong, et al. (2021). The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. American Journal of Agriculture and Forestry, 9(3), 141-146. https://doi.org/10.11648/j.ajaf.20210903.17

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    ACS Style

    Qibiao Sun; Yanfen Liu; Yan Tang; Peiyu Zhang; Yao Tong, et al. The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. Am. J. Agric. For. 2021, 9(3), 141-146. doi: 10.11648/j.ajaf.20210903.17

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    AMA Style

    Qibiao Sun, Yanfen Liu, Yan Tang, Peiyu Zhang, Yao Tong, et al. The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan. Am J Agric For. 2021;9(3):141-146. doi: 10.11648/j.ajaf.20210903.17

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  • @article{10.11648/j.ajaf.20210903.17,
      author = {Qibiao Sun and Yanfen Liu and Yan Tang and Peiyu Zhang and Yao Tong and Gang He and Xiaohong Ji and Zhenying He and Jianping Ouyang and Hongfang Zhang and Ye Chen},
      title = {The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan},
      journal = {American Journal of Agriculture and Forestry},
      volume = {9},
      number = {3},
      pages = {141-146},
      doi = {10.11648/j.ajaf.20210903.17},
      url = {https://doi.org/10.11648/j.ajaf.20210903.17},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20210903.17},
      abstract = {Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - The Composition and Phosphate-Solubilizing Capability of Phosphate-Solubilizing Bacteria in the Rhizosphere of Wild Camellia oleifera in Mountain Lushan
    AU  - Qibiao Sun
    AU  - Yanfen Liu
    AU  - Yan Tang
    AU  - Peiyu Zhang
    AU  - Yao Tong
    AU  - Gang He
    AU  - Xiaohong Ji
    AU  - Zhenying He
    AU  - Jianping Ouyang
    AU  - Hongfang Zhang
    AU  - Ye Chen
    Y1  - 2021/05/31
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ajaf.20210903.17
    DO  - 10.11648/j.ajaf.20210903.17
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 141
    EP  - 146
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20210903.17
    AB  - Camellia oleifera, a unique edible oil tree species in China, is of important economic value. However, the shortage of phosphorus in the soil is one of the important factors limiting the growth of C. oleifera. Here, we investigated the population size and composition of culturable phosphate-solubilizing bacteria (PSB) in the rhizosphere soil of wild C. oleifera in Mountain Lushan, China. PSB were isolated using a dilution coating plate method and identified by 16S rRNA sequencing. The phosphate-solubilizing capability of the isolated PSB was evaluated by a semi-quantitative method (the ratio of phosphate solubilization halo diameter versus colony diameter). The results showed that large amounts of PSB existed in the rhizosphere soil of wild C. oleifera (0.28–1.08×107 CFU/g soil) and the population size of PSB differed from investigated trees. A total of 100 strains of PSB were isolated from the rhizosphere soil, belonging to Bacillus, Burkholderia, Pantoea, Paraburkholderia, and Pseudomonas, respectively. Of these strains, Burkholderia showed the highest isolation frequency and phosphate-solubilizing capability, accounting for 61% of the isolates. The phosphate solubilization index of 100 strains varied from 1.02 to 3.04 after a 6-day incubation, and Bacillus strains were easy to lose their phosphate-solubilizing capability during the incubation. Our result suggested that Burkholderia was the dominant genus of PSB in the rhizosphere of C. oleifera and could be utilized for facilitating the uptake of P.
    VL  - 9
    IS  - 3
    ER  - 

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Author Information
  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Electronic Commerce, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

  • College of Pharmacy and Life Sciences, Jiujiang University, Jiujiang, China

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