Characterization and Cytotoxic Activity of <i>Dalbergia latifolia</i> Wood Extract

Thangamani Dhandapani; KR Sasidharan; Girija Shanmugam; Vimaladevi Sadanandam; Ragunathan Vasudevan; Suryanarayanan Lalitha; Ravichandran Anandalakshmi

doi:doi:10.11648/j.ajaf.20200805.14

Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract

Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, Suryanarayanan Lalitha, Ravichandran Anandalakshmi

Published in American Journal of Agriculture and Forestry (Volume 8, Issue 5)

Received: 19 September 2020 Accepted: 28 September 2020 Published: 12 October 2020

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Abstract

Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.

Published in	American Journal of Agriculture and Forestry (Volume 8, Issue 5)
DOI	10.11648/j.ajaf.20200805.14
Page(s)	208-213
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2020. Published by Science Publishing Group

Keywords

Dalbergia latifolia, Isoliquiritigenin, Breast Cancer Cell Line, Cytotoxicity, Conservation

References

[1]	Changliang Chen, Anitha K. Shenoy, Ravi Padia, Dongdong Fang, Qing Jing, Ping Yang, Shi-Bing Suand Shuang Huang. (2018). Suppression of lung cancer progression by isoliquiritigenin through its metabolite 2, 4, 2’, 4’-Tetrahydroxy chalcone. Journal of Experimental & Clinical Cancer Research 37: 243.
[2]	Biyan Zhang, Yun Lai, Yufeng Li, Nan Shu, Zheng Wang, Yanping Wang, Yunsen Li Zijun Chen. (2018). Antineoplastic activity of isoliquiritigenin, a chalcone compound, in androgen-independent human prostate cancer cells linked to G2/M cell cycle arrest and cell apoptosis. Europian Journal of Pharmacology. Volume 821, 15 February Pages 57-67.
[3]	H. Honda, Y. Nagai and T. Matsunaga. (2012) Glycyrrhizin and isoliquiritigenin suppress the LPS sensor toll-like receptor 4/MD-2 complex signaling in a different manner, Journal of Leukocyte Biology, vol. 91, no. 6, pp. 967–976.
[4]	Nagisa Nosrati, Marica Bakovic, Gopinadhan Paliyath. (2017). Molecular Mechanisms and Pathways as Targets for Cancer Prevention and Progression with Dietary Compounds. Int J Mol Sci, Sep 25; 18 (10): 2050.
[5]	Phamhuy, L. A. H. He, and C. Phamhuy (2008) Free radicals, antioxidants in disease and health, InternatIonal Journal of Biomedical Science, vol. 4, no. 2, pp. 89–96.
[6]	Mohammad Khalid, H. H. Siddiqui and Sheeba Freed (2011). In-vitro Assessment of Antioxidant Activity of Dalbergia latifolia Barks Extract Against Free Radicals American-Eurasian Journal of Scientific Research, 6 (3): 172-177, 2011 ISSN 1818-6785.
[7]	Sheu-Meei Yu and Sheng-Chu Kuo (1995). Vasorelaxant effect of isoliquiritigenin, a novel soluble guanylate cyclase activator, in rat aorta. British J of Pharmacology 124, 1587-1594.
[8]	Muhammad Zubair, Hilde Nybom, Christina Lindholm, Johanna M Brandner, Kimmo Rumpunen. (2016). Promotion of wound healing by Plantago major L. leaf extracts–ex-vivo experiments confirm experiences from traditional medicine, Natural product research, Volume 30,5, 622-624.
[9]	Sheu-Meei Yu and Sheng-Chu Kuo (1995). Vasorelaxant effect of isoliquiritigenin, a novel soluble guanylate cyclase activator, in rat aorta. British J of Pharmacology 124, 1587-1594.
[10]	Houghton, P., Photiou, A., Uddin, S., Shah, P., Browning, M., Jackson, S., Retsas, S (1994) Activity of extracts of Kigelia pinnata against melanoma and renal carcinoma cell lines Planta med 60 (05), pp. 430-433.
[11]	Nutan V Badgujar and Kinnari N Mistry (2017) In-vitro antioxidative, cytotoxic activity and phytochemical screening from stem bark of Kigelia pinnata" International J of Biotechnology and Biochemistry ISSN 0973-2691 Volume 13, Number 1 pp. 11-18.
[12]	Liping Ye 1, Junjie Zhang 2, Yu Zhang 1, Binbin Gu 1, Hongyuan Zhu 1, Xinli Mao. (2020) Isoliquiritigenin Suppressed Esophageal Squamous Carcinoma Growth by Blocking EGFR Activation and Inducing Cell Cycle Arrest. Biomed Res Int. 2020 Mar 8; 2020: 9259852.
[13]	Ewelina, S.; Zenon, P. C.; Bogdan, M.; Andrzej, P.; Wojciech, K. (2010) "Chalcones and Dihydrochalcones Augment TRAIL-Mediated Apoptosis in Prostate Cancer Cells" Molecules 2010, 15, 5336–5353.
[14]	Khushbu N. Thakkar, A K Prasad, Jyotiranjan Nayak, Satish V. Iyer, Shankulkumar (2014). Antioxidant and in vitro cytotoxic activity of extracts of aerial parts of Cocculushirsutus (L) using cell line cultures (breast cell line). The Journal of Phytopharmacology; 3 (6): 395-399.
[15]	Muhammad Sulaiman, Zubair, SyarifulAnam, SubehanLallo (2016). Cytotoxic activity and phytochemical standardization of Lunasiaamara Blanco wood extract. Asian Pacific Journal of Tropical Biomedicine, Volume 6, Issue 11, November 2016, Pages 962-966.
[16]	Jiabing, W. Lili, H. Chanchan, C. Ge, L. Jingwen, X. Mengya, S. Qian, C. Wulan, L., Wenfei, H. Peihong, Q. (2019) Design, synthesis and biological evaluation of chalcone analogues with novel dual antioxidant mechanisms as potential anti-ischemic stroke agents. Acta Pharm. Sin. B 2019, 9, 335–350.
[17]	Elfi, S. V. H.; Widiastuti, A. E. S (2018) A green synthesis of chalcones as an antioxidant and anticancer. IOP Conf. Ser. Mater. Sci. Eng. (2018), 299, 012077.
[18]	Cuendet, M., Guo, J., Luo, Y., Chen, S.-N., Oteham, C. P., Moon, R. C., Van Breemen, R. B., Marler, L. E., Pezzuto, J. M. (2010) Cancer chemopreventive activity and metabolism of isoliquiritigenin, a compound found in licorice. Cancer Prev. Res. 2010, 3, 221–232.
[19]	Wang, K.-L.; Hsia, S.-M.; Chan, C.-J.; Chang, F.-Y.; Huang, C.; Bau, D.-T.; Wang, P. S. (2013) Inhibitory effects of isoliquiritigenin on the migration and invasion of human breast cancer cells. Expert Opin. Ther. Targets 2013, 17, 337–349.
[20]	Chen, H.-Y.; Huang, T.-C.; Shieh, T.-M.; Wu, C.-H.; Lin, L.-C.; Hsia, S.-M. (2017) Isoliquiritigenin Induces Autophagy and Inhibits Ovarian Cancer Cell Growth. Int. J. Mol. Sci. 2017, 18, 2025.
[21]	Po-Han Li, Yi-Fen Chiang, Tzong-Ming Shieh, Hsin-Yuan Chen, Chun-Kuang Shih, Tong-Hong Wang, Kai-Lee Wang, Tsui-Chin Huan, Yong-Han Hong, Sing-Chung Li and Shih-Min Hsia (2020) Dietary Compound Isoliquiritigenin, an Antioxidant from Licorice, Suppresses Triple-Negative Breast Tumor Growth via Apoptotic Death Program Activation in Cell and Xenograft Animal Models Antioxidants 2020, 9, 228; doi: 10.3390/antiox9030228.
[22]	Peng, F. Tang, H. Liu, P Shen, J. Guan, X.-Y. Xie, X. Gao, J. Xiong, L. Jia, L. Chen, J. (2017). Isoliquiritigenin modulates miR-374a/PTEN/Akt axis to suppress breast cancer tumorigenesis and metastasis. Sci. Rep. 2017, 7, 9022.
[23]	Wang, Z.; Wang, N.; Han, S.; Wang, N.; Mo, S.; Yu, L.; Huang, H.; Tsui, K.; Shen, J.; Chen, J. 2013. Dietary Compound Isoliquiritigenin Inhibits Breast Cancer Neoangiogenesis via VEGF/VEGFR-2 Signaling Pathway. PLoS ONE, 8, e68566.
[24]	Zhang, X.; Zhu, P.; Zhang, X.; Ma, Y.; Li, W.; Chen, J.-M.; Guo, H.-M.; Bucala, R.; Zhuang, J.; Li, J. (2013) Natural Antioxidant-Isoliquiritigenin Ameliorates Contractile Dysfunction of Hypoxic Cardiomyocytes via AMPK Signaling Pathway. Mediat. Inflamm. 2013, 390890.
[25]	L. L. Mensor, F. S. Menezes, G. G. Leitao (2001)Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method, Phytotherapy Research, vol. 15, pp. 127–130, 2001.
[26]	Martinez CA, Loureiro ME, Oliva MA, Maestri M. (2001) Differential responses of superoxide dismutase in freezing resistant Solanumcurtilobumand freezing sensitive Solanumtuberosumsubjected to oxidative and water stress. Plant Sci 2001; 160: 505-15.

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APA Style

Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, et al. (2020). Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. American Journal of Agriculture and Forestry, 8(5), 208-213. https://doi.org/10.11648/j.ajaf.20200805.14

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ACS Style

Thangamani Dhandapani; KR Sasidharan; Girija Shanmugam; Vimaladevi Sadanandam; Ragunathan Vasudevan, et al. Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. Am. J. Agric. For. 2020, 8(5), 208-213. doi: 10.11648/j.ajaf.20200805.14

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AMA Style

Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, et al. Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. Am J Agric For. 2020;8(5):208-213. doi: 10.11648/j.ajaf.20200805.14

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@article{10.11648/j.ajaf.20200805.14,
  author = {Thangamani Dhandapani and KR Sasidharan and Girija Shanmugam and Vimaladevi Sadanandam and Ragunathan Vasudevan and Suryanarayanan Lalitha and Ravichandran Anandalakshmi},
  title = {Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract},
  journal = {American Journal of Agriculture and Forestry},
  volume = {8},
  number = {5},
  pages = {208-213},
  doi = {10.11648/j.ajaf.20200805.14},
  url = {https://doi.org/10.11648/j.ajaf.20200805.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20200805.14},
  abstract = {Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.},
 year = {2020}
}

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TY  - JOUR
T1  - Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract
AU  - Thangamani Dhandapani
AU  - KR Sasidharan
AU  - Girija Shanmugam
AU  - Vimaladevi Sadanandam
AU  - Ragunathan Vasudevan
AU  - Suryanarayanan Lalitha
AU  - Ravichandran Anandalakshmi
Y1  - 2020/10/12
PY  - 2020
N1  - https://doi.org/10.11648/j.ajaf.20200805.14
DO  - 10.11648/j.ajaf.20200805.14
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 208
EP  - 213
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20200805.14
AB  - Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.
VL  - 8
IS  - 5
ER  -

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Author Information

Thangamani Dhandapani

Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
KR Sasidharan

Forest Ecology and Climate Change Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Girija Shanmugam

Department of Biotechnology, Bharathiar University, Coimbatore, India
Vimaladevi Sadanandam

Germplasm Conservation Division, ICAR-NBPGR, New Delhi, India
Ragunathan Vasudevan

Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Suryanarayanan Lalitha

Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India
Ravichandran Anandalakshmi

Forest Genetic Resource Management Division, Institute of Forest Genetics and Tree Breeding, Coimbatore, India

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APA Style

Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, et al. (2020). Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. American Journal of Agriculture and Forestry, 8(5), 208-213. https://doi.org/10.11648/j.ajaf.20200805.14

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ACS Style

Thangamani Dhandapani; KR Sasidharan; Girija Shanmugam; Vimaladevi Sadanandam; Ragunathan Vasudevan, et al. Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. Am. J. Agric. For. 2020, 8(5), 208-213. doi: 10.11648/j.ajaf.20200805.14

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AMA Style

Thangamani Dhandapani, KR Sasidharan, Girija Shanmugam, Vimaladevi Sadanandam, Ragunathan Vasudevan, et al. Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract. Am J Agric For. 2020;8(5):208-213. doi: 10.11648/j.ajaf.20200805.14

Copy | Download

@article{10.11648/j.ajaf.20200805.14,
  author = {Thangamani Dhandapani and KR Sasidharan and Girija Shanmugam and Vimaladevi Sadanandam and Ragunathan Vasudevan and Suryanarayanan Lalitha and Ravichandran Anandalakshmi},
  title = {Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract},
  journal = {American Journal of Agriculture and Forestry},
  volume = {8},
  number = {5},
  pages = {208-213},
  doi = {10.11648/j.ajaf.20200805.14},
  url = {https://doi.org/10.11648/j.ajaf.20200805.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20200805.14},
  abstract = {Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.},
 year = {2020}
}

Copy | Download

TY  - JOUR
T1  - Characterization and Cytotoxic Activity of Dalbergia latifolia Wood Extract
AU  - Thangamani Dhandapani
AU  - KR Sasidharan
AU  - Girija Shanmugam
AU  - Vimaladevi Sadanandam
AU  - Ragunathan Vasudevan
AU  - Suryanarayanan Lalitha
AU  - Ravichandran Anandalakshmi
Y1  - 2020/10/12
PY  - 2020
N1  - https://doi.org/10.11648/j.ajaf.20200805.14
DO  - 10.11648/j.ajaf.20200805.14
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 208
EP  - 213
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20200805.14
AB  - Dalbergia latifolia commonly known as the Indian rosewood, commonly found in association with Teak trees population in and around Western Ghat areas of Tamil Nadu and Kerala in India. From the heartwood of roots and trunk many phytochemical contents were investigated. A wide spectrum of flavanoids namely, flavones, isoflavones, neoflavones and chalcones have been obtained. Specifically, one of the flavonoids compound chalcone compound-Isoliquiritigenin (ILTG, 2',4',4'-trihydroxychalcone) shows various pharmacological properties, including cytotoxic activities. This study revealed the cytotoxic effect of Isoliquiritigenin, efficiently controls the invasive capacity of breast cancer cells MCF10A human mammary epithelial cell line through uplifting G1-phase cell cycle, even arrest at low concentration, also accelerate the extracellular signal regulated kinase signaling pathway to topup the proteins combined with apoptosis and arrest cancer. The cytotoxicity invitro of isoliquitrigenin against MCF10A ATCC cells was investigated by MTT assay. Apoptosis was evaluated by microscopy and flow cytometry. Isoliquiritigenin exhibited the most potent anticancer activity against MCF 10A ATCC with an IC 50 of 10.00 µg/ml. The methanolic extract expressed cytotoxic effect with an IC50 value of 20 µg/ml. The compounds were also tested for their toxicity on normal human cell lines-LO2 and were found to be nontoxic. Administering the wood extract approaches may help reduce side effects in patients under conventional chemotherapy. These potential molecule may exerts their co-active effects through various pathways and helpful for the development of novel drugs; also conservation of these important germplasm is mandatory.
VL  - 8
IS  - 5
ER  -

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