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Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice

Received: 7 April 2018     Accepted: 23 April 2018     Published: 11 May 2018
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Abstract

A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.

Published in American Journal of Agriculture and Forestry (Volume 6, Issue 2)
DOI 10.11648/j.ajaf.20180602.11
Page(s) 18-27
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2018. Published by Science Publishing Group

Keywords

Rice, OsWD1, Overexpression, Plant Height, Grain Size, Gibberellin Acid

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Cite This Article
  • APA Style

    Siou-Luan He, Shin-Lon Ho. (2018). Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. American Journal of Agriculture and Forestry, 6(2), 18-27. https://doi.org/10.11648/j.ajaf.20180602.11

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    ACS Style

    Siou-Luan He; Shin-Lon Ho. Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. Am. J. Agric. For. 2018, 6(2), 18-27. doi: 10.11648/j.ajaf.20180602.11

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    AMA Style

    Siou-Luan He, Shin-Lon Ho. Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice. Am J Agric For. 2018;6(2):18-27. doi: 10.11648/j.ajaf.20180602.11

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  • @article{10.11648/j.ajaf.20180602.11,
      author = {Siou-Luan He and Shin-Lon Ho},
      title = {Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice},
      journal = {American Journal of Agriculture and Forestry},
      volume = {6},
      number = {2},
      pages = {18-27},
      doi = {10.11648/j.ajaf.20180602.11},
      url = {https://doi.org/10.11648/j.ajaf.20180602.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20180602.11},
      abstract = {A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.},
     year = {2018}
    }
    

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  • TY  - JOUR
    T1  - Functional Characterization of a WD-Repeat Protein Gene (OsWD1) in Rice
    AU  - Siou-Luan He
    AU  - Shin-Lon Ho
    Y1  - 2018/05/11
    PY  - 2018
    N1  - https://doi.org/10.11648/j.ajaf.20180602.11
    DO  - 10.11648/j.ajaf.20180602.11
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 18
    EP  - 27
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20180602.11
    AB  - A gene encoded WD-40 repeat protein (OsWD1) was isolated from rice cells to study the platform for protein-protein interactions in germinating seeds. The expression of OsWD1 was found to be specifically induced at 3 d of seed germination. By ectopic overexpressing (OsWD1-Ox) or RNA interference gene knockdown (OsWD1-Ri) of OsWD1, respectively. Results revealed that the rice seedlings and grain size were enhanced in OsWD1-Ox lines, but were reduced in OsWD1-Ri lines. Moreover, the expression of GA-inducible gene, OsEP3A, which was strongly induced in OsWD1-Ox lines during rice seed germination. By iodine staining in starch containing solid medium, the a-amylase activities were further enhanced in OsWD1-Ox lines regardless with or without exogenous GA in the medium. These results suggested that OsWD1 might acts as a platform for protein-protein interactions to regulate seedling and seed development in rice by a GA-dependent manner.
    VL  - 6
    IS  - 2
    ER  - 

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Author Information
  • Department of Agronomy, National Chiayi University, Chiayi, Taiwan, R.O.C.

  • Department of Agronomy, National Chiayi University, Chiayi, Taiwan, R.O.C.

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