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Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers

Received: 25 May 2016     Accepted: 12 June 2016     Published: 23 July 2016
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Abstract

Ceiba pentandra (L.) Gaertn (trade name Ceiba) of the family Bombacaceae is an important multi-purpose tree species in Ghana and demand for it is rising daily. As a result, it has been included as one of the species for the National Forest Plantation Development Programme of Ghana as part of the efforts towards its restoration. Seedlings for the programme are being raised from seed collected from the wild without regard for their genetic quality and its future adverse effects. The absence of adequate information on the genetic diversity within the species therefore serves as a potential threat to its long term sustainable management and efficient genetic conversation. The phylogenetic relationships of 36 genotypes of C. pentandra from natural range of the species in five ecological zones (populations) of Ghana were therefore analyzed using 10 polymerase chain reaction (PCR) markers (5 random amplified polymorphic DNAs (RAPDs) and 5 inter simple sequence repeats (ISSRs)). The principal component analysis (PcoA) defined by axis 1 and 2 accounted for 67.15% of the variation observed. Cluster analysis using GeneStat Discovery showed that, 14 (38.89%), 21 (58.33%) and 4 (11.11%) of the accessions had a coefficient of similarity of 1 from the RAPD, ISSR and the combined RAPD and ISSR polymorphism respectively. The most distantly related accessions from the RAPD polymorphism analysis were BAW 1 and KON 8 with a similarity coefficient of 0.06 whereas those from the ISSR polymorphism were BAW 10 and ASE 1 with 21% similarity between them. Similarly, BUF 1 and KUE 1 were the most dissimilar accessions from the combined RAPD and ISSR fragments analysis with a similarity coefficient of 0.23. Nine (25%) accessions with high degree of dissimilarity between them were identified in the study. These accessions could serve as good candidates for conservation as seed trees and in breeding programs of C. pentandra in Ghana. Implications of the findings for effective management of the genetic resources of the species were also discussed.

Published in American Journal of Agriculture and Forestry (Volume 4, Issue 4)
DOI 10.11648/j.ajaf.20160404.13
Page(s) 86-96
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2016. Published by Science Publishing Group

Keywords

Ceiba pentandra (L.), ISSR, RAPD, Genetic Diversity, Population, Genotypes

References
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    Cosmas Sorngmenenye Abengmeneng, Daniel Ofori, Philomena Kumapley, Richard Akromah, Ramni Jamnadass, et al. (2016). Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers. American Journal of Agriculture and Forestry, 4(4), 86-96. https://doi.org/10.11648/j.ajaf.20160404.13

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    ACS Style

    Cosmas Sorngmenenye Abengmeneng; Daniel Ofori; Philomena Kumapley; Richard Akromah; Ramni Jamnadass, et al. Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers. Am. J. Agric. For. 2016, 4(4), 86-96. doi: 10.11648/j.ajaf.20160404.13

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    AMA Style

    Cosmas Sorngmenenye Abengmeneng, Daniel Ofori, Philomena Kumapley, Richard Akromah, Ramni Jamnadass, et al. Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers. Am J Agric For. 2016;4(4):86-96. doi: 10.11648/j.ajaf.20160404.13

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  • @article{10.11648/j.ajaf.20160404.13,
      author = {Cosmas Sorngmenenye Abengmeneng and Daniel Ofori and Philomena Kumapley and Richard Akromah and Ramni Jamnadass and Marian Quain},
      title = {Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers},
      journal = {American Journal of Agriculture and Forestry},
      volume = {4},
      number = {4},
      pages = {86-96},
      doi = {10.11648/j.ajaf.20160404.13},
      url = {https://doi.org/10.11648/j.ajaf.20160404.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20160404.13},
      abstract = {Ceiba pentandra (L.) Gaertn (trade name Ceiba) of the family Bombacaceae is an important multi-purpose tree species in Ghana and demand for it is rising daily. As a result, it has been included as one of the species for the National Forest Plantation Development Programme of Ghana as part of the efforts towards its restoration. Seedlings for the programme are being raised from seed collected from the wild without regard for their genetic quality and its future adverse effects. The absence of adequate information on the genetic diversity within the species therefore serves as a potential threat to its long term sustainable management and efficient genetic conversation. The phylogenetic relationships of 36 genotypes of C. pentandra from natural range of the species in five ecological zones (populations) of Ghana were therefore analyzed using 10 polymerase chain reaction (PCR) markers (5 random amplified polymorphic DNAs (RAPDs) and 5 inter simple sequence repeats (ISSRs)). The principal component analysis (PcoA) defined by axis 1 and 2 accounted for 67.15% of the variation observed. Cluster analysis using GeneStat Discovery showed that, 14 (38.89%), 21 (58.33%) and 4 (11.11%) of the accessions had a coefficient of similarity of 1 from the RAPD, ISSR and the combined RAPD and ISSR polymorphism respectively. The most distantly related accessions from the RAPD polymorphism analysis were BAW 1 and KON 8 with a similarity coefficient of 0.06 whereas those from the ISSR polymorphism were BAW 10 and ASE 1 with 21% similarity between them. Similarly, BUF 1 and KUE 1 were the most dissimilar accessions from the combined RAPD and ISSR fragments analysis with a similarity coefficient of 0.23. Nine (25%) accessions with high degree of dissimilarity between them were identified in the study. These accessions could serve as good candidates for conservation as seed trees and in breeding programs of C. pentandra in Ghana. Implications of the findings for effective management of the genetic resources of the species were also discussed.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Genetic Relationship Among 36 Genotypes of Ceiba pentandra (L.) as Revealed by RAPD and ISSR Markers
    AU  - Cosmas Sorngmenenye Abengmeneng
    AU  - Daniel Ofori
    AU  - Philomena Kumapley
    AU  - Richard Akromah
    AU  - Ramni Jamnadass
    AU  - Marian Quain
    Y1  - 2016/07/23
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ajaf.20160404.13
    DO  - 10.11648/j.ajaf.20160404.13
    T2  - American Journal of Agriculture and Forestry
    JF  - American Journal of Agriculture and Forestry
    JO  - American Journal of Agriculture and Forestry
    SP  - 86
    EP  - 96
    PB  - Science Publishing Group
    SN  - 2330-8591
    UR  - https://doi.org/10.11648/j.ajaf.20160404.13
    AB  - Ceiba pentandra (L.) Gaertn (trade name Ceiba) of the family Bombacaceae is an important multi-purpose tree species in Ghana and demand for it is rising daily. As a result, it has been included as one of the species for the National Forest Plantation Development Programme of Ghana as part of the efforts towards its restoration. Seedlings for the programme are being raised from seed collected from the wild without regard for their genetic quality and its future adverse effects. The absence of adequate information on the genetic diversity within the species therefore serves as a potential threat to its long term sustainable management and efficient genetic conversation. The phylogenetic relationships of 36 genotypes of C. pentandra from natural range of the species in five ecological zones (populations) of Ghana were therefore analyzed using 10 polymerase chain reaction (PCR) markers (5 random amplified polymorphic DNAs (RAPDs) and 5 inter simple sequence repeats (ISSRs)). The principal component analysis (PcoA) defined by axis 1 and 2 accounted for 67.15% of the variation observed. Cluster analysis using GeneStat Discovery showed that, 14 (38.89%), 21 (58.33%) and 4 (11.11%) of the accessions had a coefficient of similarity of 1 from the RAPD, ISSR and the combined RAPD and ISSR polymorphism respectively. The most distantly related accessions from the RAPD polymorphism analysis were BAW 1 and KON 8 with a similarity coefficient of 0.06 whereas those from the ISSR polymorphism were BAW 10 and ASE 1 with 21% similarity between them. Similarly, BUF 1 and KUE 1 were the most dissimilar accessions from the combined RAPD and ISSR fragments analysis with a similarity coefficient of 0.23. Nine (25%) accessions with high degree of dissimilarity between them were identified in the study. These accessions could serve as good candidates for conservation as seed trees and in breeding programs of C. pentandra in Ghana. Implications of the findings for effective management of the genetic resources of the species were also discussed.
    VL  - 4
    IS  - 4
    ER  - 

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Author Information
  • Department of Silviculture and Forest Management, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • Forestry Research Institute of Ghana, Council for Scientific and Industrial Research, Kumasi, Ghana

  • Department of Silviculture and Forest Management, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • Department of Crop and Soil Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana

  • International Centre for Research into Agroforestry, Nairobi, Kenya

  • Crop Research Institute, Council for Scientific and Industrial Research, Kumasi, Ghana

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