Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato

Md. Afzal Hossain; Md. Abu Kawochar; Abdullah-Al-Mahmud; Ebna Habib Md. Shofiur Rahaman; Md. Altaf Hossain; Khondoker Md. Nasiruddin

doi:doi:10.11648/j.ajaf.20150302.13

Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato

Md. Afzal Hossain, Md. Abu Kawochar, Abdullah-Al-Mahmud, Ebna Habib Md. Shofiur Rahaman, Md. Altaf Hossain, Khondoker Md. Nasiruddin

Published in American Journal of Agriculture and Forestry (Volume 3, Issue 2)

Received: 25 January 2015 Accepted: 10 February 2015 Published: 8 March 2015

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Abstract

In vitro micro tuberization from regenerated plantlets of potato varieties was observed in Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University. Tubers of potato cultivars Diamant and Cardinal were used as initial experimental materials for meristem culture. The experiment was consisted of three factors; variety (Diamant, Cardinal), sucrose concentration (3%, 6%, 9%, 12%) and 6-benzyl aminopurine (BAP) levels (2.5, 5.0, 7.5 mg L-1). As a whole, 24 treatments were laid out in complete randomized design with three replications. Among the varieties, Diamant required minimum days (6-17) for micro-tuber initiation, produced more number of micro-tubers (4.97) and produced more average weight of micro-tuber (120.39mg) but there had no significant difference. Among the sucrose levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.06) and the highest average weight of micro-tuber (137.31 mg) were found in 9% sucrose level. For different BAP levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.38) and the highest average weight of micro-tuber (126.31 mg) were found at 5.0 mg L-1. The best combination for minimum duration (6-8 days) of micro-tuber initiation, the highest number of micro-tubers vial-1 (6.00) and the highest average weight of micro-tuber (152.01 mg) was in Diamant with 9% sucrose at 5 mg L-1. Concomitantly, the lowest number micro-tubers vial-1 (2.00) and the lowest average weight of micro-tuber (89.98 mg) were found in Cardinal cultured with 3% sucrose media where at 7.5 mg L-1 BAP and at 2.5 mg L-1 BAP, respectively.

Published in	American Journal of Agriculture and Forestry (Volume 3, Issue 2)
DOI	10.11648/j.ajaf.20150302.13
Page(s)	25-30
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

6-Benzyl Aminopurine, Microtuberization, Plantlets, Potato

References

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[3]	Cui, C., F. F. F. He, J. C. Wang, Q. Y. Zhou, Y. X. Huang and D. B. Tang. 2001. Effects of photoperiod and carbon sources on the formation of microtubers of potato in vitro. J. Southwest Agril. Varsity. 23: 547-548.
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[13]	Liu, Z. L., Z. L. Wang, S. L. Wang, K. Gao. 2002. Effects of chlorobromoisocyanuric acid on culture of potato (Solanum tuberosum) plantlets in vitro. Chinese Potato J. 16(5): 288-290.
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Md. Afzal Hossain, Md. Abu Kawochar, Abdullah-Al-Mahmud, Ebna Habib Md. Shofiur Rahaman, Md. Altaf Hossain, et al. (2015). Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato. American Journal of Agriculture and Forestry, 3(2), 25-30. https://doi.org/10.11648/j.ajaf.20150302.13

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ACS Style

Md. Afzal Hossain; Md. Abu Kawochar; Abdullah-Al-Mahmud; Ebna Habib Md. Shofiur Rahaman; Md. Altaf Hossain, et al. Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato. Am. J. Agric. For. 2015, 3(2), 25-30. doi: 10.11648/j.ajaf.20150302.13

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Md. Afzal Hossain, Md. Abu Kawochar, Abdullah-Al-Mahmud, Ebna Habib Md. Shofiur Rahaman, Md. Altaf Hossain, et al. Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato. Am J Agric For. 2015;3(2):25-30. doi: 10.11648/j.ajaf.20150302.13

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@article{10.11648/j.ajaf.20150302.13,
  author = {Md. Afzal Hossain and Md. Abu Kawochar and Abdullah-Al-Mahmud and Ebna Habib Md. Shofiur Rahaman and Md. Altaf Hossain and Khondoker Md. Nasiruddin},
  title = {Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato},
  journal = {American Journal of Agriculture and Forestry},
  volume = {3},
  number = {2},
  pages = {25-30},
  doi = {10.11648/j.ajaf.20150302.13},
  url = {https://doi.org/10.11648/j.ajaf.20150302.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20150302.13},
  abstract = {In vitro micro tuberization from regenerated plantlets of potato varieties was observed in Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University. Tubers of potato cultivars Diamant and Cardinal were used as initial experimental materials for meristem culture. The experiment was consisted of three factors; variety (Diamant, Cardinal), sucrose concentration (3%, 6%, 9%, 12%) and 6-benzyl aminopurine (BAP) levels (2.5, 5.0, 7.5 mg L-1). As a whole, 24 treatments were laid out in complete randomized design with three replications. Among the varieties, Diamant required minimum days (6-17) for micro-tuber initiation, produced more number of micro-tubers (4.97) and produced more average weight of micro-tuber (120.39mg) but there had no significant difference. Among the sucrose levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.06) and the highest average weight of micro-tuber (137.31 mg) were found in 9% sucrose level. For different BAP levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.38) and the highest average weight of micro-tuber (126.31 mg) were found at 5.0 mg L-1. The best combination for minimum duration (6-8 days) of micro-tuber initiation, the highest number of micro-tubers vial-1 (6.00) and the highest average weight of micro-tuber (152.01 mg) was in Diamant with 9% sucrose at 5 mg L-1. Concomitantly, the lowest number micro-tubers vial-1 (2.00) and the lowest average weight of micro-tuber (89.98 mg) were found in Cardinal cultured with 3% sucrose media where at 7.5 mg L-1 BAP and at 2.5 mg L-1 BAP, respectively.},
 year = {2015}
}

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TY  - JOUR
T1  - Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato
AU  - Md. Afzal Hossain
AU  - Md. Abu Kawochar
AU  - Abdullah-Al-Mahmud
AU  - Ebna Habib Md. Shofiur Rahaman
AU  - Md. Altaf Hossain
AU  - Khondoker Md. Nasiruddin
Y1  - 2015/03/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajaf.20150302.13
DO  - 10.11648/j.ajaf.20150302.13
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 25
EP  - 30
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20150302.13
AB  - In vitro micro tuberization from regenerated plantlets of potato varieties was observed in Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University. Tubers of potato cultivars Diamant and Cardinal were used as initial experimental materials for meristem culture. The experiment was consisted of three factors; variety (Diamant, Cardinal), sucrose concentration (3%, 6%, 9%, 12%) and 6-benzyl aminopurine (BAP) levels (2.5, 5.0, 7.5 mg L-1). As a whole, 24 treatments were laid out in complete randomized design with three replications. Among the varieties, Diamant required minimum days (6-17) for micro-tuber initiation, produced more number of micro-tubers (4.97) and produced more average weight of micro-tuber (120.39mg) but there had no significant difference. Among the sucrose levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.06) and the highest average weight of micro-tuber (137.31 mg) were found in 9% sucrose level. For different BAP levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.38) and the highest average weight of micro-tuber (126.31 mg) were found at 5.0 mg L-1. The best combination for minimum duration (6-8 days) of micro-tuber initiation, the highest number of micro-tubers vial-1 (6.00) and the highest average weight of micro-tuber (152.01 mg) was in Diamant with 9% sucrose at 5 mg L-1. Concomitantly, the lowest number micro-tubers vial-1 (2.00) and the lowest average weight of micro-tuber (89.98 mg) were found in Cardinal cultured with 3% sucrose media where at 7.5 mg L-1 BAP and at 2.5 mg L-1 BAP, respectively.
VL  - 3
IS  - 2
ER  -

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Author Information

Md. Afzal Hossain

Ministry of Environment & Forests, Bangladesh Secretariat, Bangladesh
Md. Abu Kawochar

Tuber crops research Centre (TCRC), Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
Abdullah-Al-Mahmud

International Potato Centre (CIP), SWCA, Banani, Dhaka, Bangladesh
Ebna Habib Md. Shofiur Rahaman

International Potato Centre (CIP), SWCA, Banani, Dhaka, Bangladesh
Md. Altaf Hossain

Tuber crops research Centre (TCRC), Bangladesh Agricultural Research Institute, Joydebpur, Gazipur, Bangladesh
Khondoker Md. Nasiruddin

Department of Biotechnology, Bangladesh Agricultural University, Bangladesh

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APA Style

Md. Afzal Hossain, Md. Abu Kawochar, Abdullah-Al-Mahmud, Ebna Habib Md. Shofiur Rahaman, Md. Altaf Hossain, et al. (2015). Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato. American Journal of Agriculture and Forestry, 3(2), 25-30. https://doi.org/10.11648/j.ajaf.20150302.13

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ACS Style

Md. Afzal Hossain; Md. Abu Kawochar; Abdullah-Al-Mahmud; Ebna Habib Md. Shofiur Rahaman; Md. Altaf Hossain, et al. Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato. Am. J. Agric. For. 2015, 3(2), 25-30. doi: 10.11648/j.ajaf.20150302.13

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AMA Style

Md. Afzal Hossain, Md. Abu Kawochar, Abdullah-Al-Mahmud, Ebna Habib Md. Shofiur Rahaman, Md. Altaf Hossain, et al. Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato. Am J Agric For. 2015;3(2):25-30. doi: 10.11648/j.ajaf.20150302.13

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@article{10.11648/j.ajaf.20150302.13,
  author = {Md. Afzal Hossain and Md. Abu Kawochar and Abdullah-Al-Mahmud and Ebna Habib Md. Shofiur Rahaman and Md. Altaf Hossain and Khondoker Md. Nasiruddin},
  title = {Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato},
  journal = {American Journal of Agriculture and Forestry},
  volume = {3},
  number = {2},
  pages = {25-30},
  doi = {10.11648/j.ajaf.20150302.13},
  url = {https://doi.org/10.11648/j.ajaf.20150302.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajaf.20150302.13},
  abstract = {In vitro micro tuberization from regenerated plantlets of potato varieties was observed in Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University. Tubers of potato cultivars Diamant and Cardinal were used as initial experimental materials for meristem culture. The experiment was consisted of three factors; variety (Diamant, Cardinal), sucrose concentration (3%, 6%, 9%, 12%) and 6-benzyl aminopurine (BAP) levels (2.5, 5.0, 7.5 mg L-1). As a whole, 24 treatments were laid out in complete randomized design with three replications. Among the varieties, Diamant required minimum days (6-17) for micro-tuber initiation, produced more number of micro-tubers (4.97) and produced more average weight of micro-tuber (120.39mg) but there had no significant difference. Among the sucrose levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.06) and the highest average weight of micro-tuber (137.31 mg) were found in 9% sucrose level. For different BAP levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.38) and the highest average weight of micro-tuber (126.31 mg) were found at 5.0 mg L-1. The best combination for minimum duration (6-8 days) of micro-tuber initiation, the highest number of micro-tubers vial-1 (6.00) and the highest average weight of micro-tuber (152.01 mg) was in Diamant with 9% sucrose at 5 mg L-1. Concomitantly, the lowest number micro-tubers vial-1 (2.00) and the lowest average weight of micro-tuber (89.98 mg) were found in Cardinal cultured with 3% sucrose media where at 7.5 mg L-1 BAP and at 2.5 mg L-1 BAP, respectively.},
 year = {2015}
}

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TY  - JOUR
T1  - Standardization of Sucrose and 6-Benzyl Aminopurine for in vitro Micro Tuberization of Potato
AU  - Md. Afzal Hossain
AU  - Md. Abu Kawochar
AU  - Abdullah-Al-Mahmud
AU  - Ebna Habib Md. Shofiur Rahaman
AU  - Md. Altaf Hossain
AU  - Khondoker Md. Nasiruddin
Y1  - 2015/03/08
PY  - 2015
N1  - https://doi.org/10.11648/j.ajaf.20150302.13
DO  - 10.11648/j.ajaf.20150302.13
T2  - American Journal of Agriculture and Forestry
JF  - American Journal of Agriculture and Forestry
JO  - American Journal of Agriculture and Forestry
SP  - 25
EP  - 30
PB  - Science Publishing Group
SN  - 2330-8591
UR  - https://doi.org/10.11648/j.ajaf.20150302.13
AB  - In vitro micro tuberization from regenerated plantlets of potato varieties was observed in Biotechnology Laboratory, Department of Biotechnology, Bangladesh Agricultural University. Tubers of potato cultivars Diamant and Cardinal were used as initial experimental materials for meristem culture. The experiment was consisted of three factors; variety (Diamant, Cardinal), sucrose concentration (3%, 6%, 9%, 12%) and 6-benzyl aminopurine (BAP) levels (2.5, 5.0, 7.5 mg L-1). As a whole, 24 treatments were laid out in complete randomized design with three replications. Among the varieties, Diamant required minimum days (6-17) for micro-tuber initiation, produced more number of micro-tubers (4.97) and produced more average weight of micro-tuber (120.39mg) but there had no significant difference. Among the sucrose levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.06) and the highest average weight of micro-tuber (137.31 mg) were found in 9% sucrose level. For different BAP levels, quickest (6-15 days) micro-tuber initiation, the highest number of micro-tubers vial-1 (5.38) and the highest average weight of micro-tuber (126.31 mg) were found at 5.0 mg L-1. The best combination for minimum duration (6-8 days) of micro-tuber initiation, the highest number of micro-tubers vial-1 (6.00) and the highest average weight of micro-tuber (152.01 mg) was in Diamant with 9% sucrose at 5 mg L-1. Concomitantly, the lowest number micro-tubers vial-1 (2.00) and the lowest average weight of micro-tuber (89.98 mg) were found in Cardinal cultured with 3% sucrose media where at 7.5 mg L-1 BAP and at 2.5 mg L-1 BAP, respectively.
VL  - 3
IS  - 2
ER  -

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