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Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds

Received: 31 March 2021     Accepted: 19 April 2021     Published: 24 August 2021
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Abstract

Antibiotics are of great importance in medicine, that are harmful to the growth and metabolic activities of bacteria. Ever increasing search is going on in the hope that agents’ superior to these and other antibiotics now in use might be isolated. Present study seeks to screen bacteria with antibiotic producing potentials from termite mounds. Eight (8) termite mound samples were collected from the study sites (Shamawa, Dundaye, G/Yaro and Gumburawa), processed and bacteria were isolated and identified using culturing methods, gram staining and biochemical characterization methods. The isolates were further tested for antibiotic production using crowded plate techniques on muellar-hilton agar. Morphological and cultural studies shows that twenty gram positive and six gram negative bacteria species were identified namely Bacillus spp, Citrobacter freundii, Enterococcus cloacae, Enterococcus faecalis, E. coli, K-pneumoniae, Pseudomonas spp, Staph aureus, Staph lentus and Staph ureae. Eleven bacteria species out of the fourteen isolates showed antibiotic production activity against Staphylococcus epidermidis and 3 were not active against the test organism. These include E. coli and Pseudomonas spp. The zone of inhibition formed are Bacillus cereus 4 mm, Bacillus subtilis 5 mm, Bacillus megaterium 2 mm, Citrobacter freundii 4 mm, Enterococcus cloacae 2 mm, and Enterococcus faecalis 2 mm respectively. The result of this research indicates that termites mound may be used as a source of antibiotic producing bacteria.

Published in Advances in Biochemistry (Volume 9, Issue 3)
DOI 10.11648/j.ab.20210903.14
Page(s) 56-59
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2021. Published by Science Publishing Group

Keywords

Bacillus Spp, Pseudomonas Spp, Staphylococcus Spp, Enterococus Spp

References
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[2] Baltz, R. H. (2008). Renaissance in Antibacterial Descovery From Actinomycetes. Current Opinion in Pharmacology, 8: 557-563.
[3] Berdy, J. (2001) Recent Development of Antibiotic Research and Classification of Antibiotics According to Chemical Structure. Advances in Applied Microbiology 18: 309 – 406.
[4] Berdy, J. (2004) Recent Advances in Prospects of Antibiotic Research Process Biochemistry 15: 28-35.
[5] Cheesbrough, M. (2006). Medical Laboratory Manual for Tropical Countries, 11 Microbiology (Tropical Health Technology Press edition England) 58–67.
[6] Clardy J., Fischbach, M. A. and Walsh, C. T. (2006). New Antibiotics from Bacterial Natural Products. Nat Biotech. 24 (2): 1541-1550.
[7] Casida LE. Industrial Microbiology, 3rd edition. Wiley Easter Ltd., 1984; pp 3-437.
[8] Haque SFK, Sen SK, and Pal SC. Screening and identification of antibiotic producing strains of Streptomyces. Hindustan Antibiot Bull 1992; (3-4): 76-83.
[9] Haque SK, Sen SK, Pal SC. Antimicrobial spectra and toxicity of antibiotics From Streptomyces antibioticus sr 15-4. Indian J. Microbiol. 1996; 36: 113-114.
[10] Hammond, S. M. and Lambert, P. A. (2007). Antibiotics and Antimicrobial Action London: Edward Arnold pp 5-52.
[11] Okarfor, N. (2002). Antibiotics and Anti-tumor Agents. Industrial Microbiology University of UK Press Ltd: pp 336-369.
[12] Singh, U. R., SIGH, J. and Singh, I. D. (2007). Microbial Association with Termites in a Tropical Deciduous Forest at Varanasi Tropical Ecology 19 (3): 163-173.
[13] Sun, J. Z. and Scharf, M. E. (2010). Exploring and Integrating Cellulolytic Systems of Insects to Advanced Biofuel Technology, Insect Science 17: 163-165.
[14] Skold, O. (2006). Antibiotika Och antibiotika Resistents 1th Edition Student Literature ISBN 978-9144-03621-2.
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  • APA Style

    Salisu Hussaini, Muhammad Ummulkusum, Manga Shuaibu Bala, Maimuna Abdulrahman Ashafa. (2021). Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds. Advances in Biochemistry, 9(3), 56-59. https://doi.org/10.11648/j.ab.20210903.14

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    ACS Style

    Salisu Hussaini; Muhammad Ummulkusum; Manga Shuaibu Bala; Maimuna Abdulrahman Ashafa. Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds. Adv. Biochem. 2021, 9(3), 56-59. doi: 10.11648/j.ab.20210903.14

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    AMA Style

    Salisu Hussaini, Muhammad Ummulkusum, Manga Shuaibu Bala, Maimuna Abdulrahman Ashafa. Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds. Adv Biochem. 2021;9(3):56-59. doi: 10.11648/j.ab.20210903.14

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  • @article{10.11648/j.ab.20210903.14,
      author = {Salisu Hussaini and Muhammad Ummulkusum and Manga Shuaibu Bala and Maimuna Abdulrahman Ashafa},
      title = {Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds},
      journal = {Advances in Biochemistry},
      volume = {9},
      number = {3},
      pages = {56-59},
      doi = {10.11648/j.ab.20210903.14},
      url = {https://doi.org/10.11648/j.ab.20210903.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20210903.14},
      abstract = {Antibiotics are of great importance in medicine, that are harmful to the growth and metabolic activities of bacteria. Ever increasing search is going on in the hope that agents’ superior to these and other antibiotics now in use might be isolated. Present study seeks to screen bacteria with antibiotic producing potentials from termite mounds. Eight (8) termite mound samples were collected from the study sites (Shamawa, Dundaye, G/Yaro and Gumburawa), processed and bacteria were isolated and identified using culturing methods, gram staining and biochemical characterization methods. The isolates were further tested for antibiotic production using crowded plate techniques on muellar-hilton agar. Morphological and cultural studies shows that twenty gram positive and six gram negative bacteria species were identified namely Bacillus spp, Citrobacter freundii, Enterococcus cloacae, Enterococcus faecalis, E. coli, K-pneumoniae, Pseudomonas spp, Staph aureus, Staph lentus and Staph ureae. Eleven bacteria species out of the fourteen isolates showed antibiotic production activity against Staphylococcus epidermidis and 3 were not active against the test organism. These include E. coli and Pseudomonas spp. The zone of inhibition formed are Bacillus cereus 4 mm, Bacillus subtilis 5 mm, Bacillus megaterium 2 mm, Citrobacter freundii 4 mm, Enterococcus cloacae 2 mm, and Enterococcus faecalis 2 mm respectively. The result of this research indicates that termites mound may be used as a source of antibiotic producing bacteria.},
     year = {2021}
    }
    

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  • TY  - JOUR
    T1  - Isolation, Identification and Screening of Bacteria with Antibiotic Production Potential from Termite Mounds
    AU  - Salisu Hussaini
    AU  - Muhammad Ummulkusum
    AU  - Manga Shuaibu Bala
    AU  - Maimuna Abdulrahman Ashafa
    Y1  - 2021/08/24
    PY  - 2021
    N1  - https://doi.org/10.11648/j.ab.20210903.14
    DO  - 10.11648/j.ab.20210903.14
    T2  - Advances in Biochemistry
    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
    SP  - 56
    EP  - 59
    PB  - Science Publishing Group
    SN  - 2329-0862
    UR  - https://doi.org/10.11648/j.ab.20210903.14
    AB  - Antibiotics are of great importance in medicine, that are harmful to the growth and metabolic activities of bacteria. Ever increasing search is going on in the hope that agents’ superior to these and other antibiotics now in use might be isolated. Present study seeks to screen bacteria with antibiotic producing potentials from termite mounds. Eight (8) termite mound samples were collected from the study sites (Shamawa, Dundaye, G/Yaro and Gumburawa), processed and bacteria were isolated and identified using culturing methods, gram staining and biochemical characterization methods. The isolates were further tested for antibiotic production using crowded plate techniques on muellar-hilton agar. Morphological and cultural studies shows that twenty gram positive and six gram negative bacteria species were identified namely Bacillus spp, Citrobacter freundii, Enterococcus cloacae, Enterococcus faecalis, E. coli, K-pneumoniae, Pseudomonas spp, Staph aureus, Staph lentus and Staph ureae. Eleven bacteria species out of the fourteen isolates showed antibiotic production activity against Staphylococcus epidermidis and 3 were not active against the test organism. These include E. coli and Pseudomonas spp. The zone of inhibition formed are Bacillus cereus 4 mm, Bacillus subtilis 5 mm, Bacillus megaterium 2 mm, Citrobacter freundii 4 mm, Enterococcus cloacae 2 mm, and Enterococcus faecalis 2 mm respectively. The result of this research indicates that termites mound may be used as a source of antibiotic producing bacteria.
    VL  - 9
    IS  - 3
    ER  - 

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Author Information
  • Department of Microbiology, Faculty of Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Microbiology, Faculty of Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Microbiology, Faculty of Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

  • Department of Microbiology, Faculty of Sciences, Usmanu Danfodiyo University, Sokoto, Nigeria

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