Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Daphne Santoro Leonardi-Carvalho; Sergio Zucoloto; Paula Payão Ovidio; Renato Heidor; Thomas Prates Ong; Fernando Salvador Moreno; Alceu Afonso Jordao

doi:doi:10.11648/j.ab.20150306.14

Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats

Daphne Santoro Leonardi-Carvalho, Sergio Zucoloto, Paula Payão Ovidio, Renato Heidor, Thomas Prates Ong, Fernando Salvador Moreno, Alceu Afonso Jordao

Published in Advances in Biochemistry (Volume 3, Issue 6)

Received: 31 August 2015 Accepted: 29 October 2015 Published: 10 December 2015

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Abstract

The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p<0.05). The hepatic cholesterol concentration was highest in the HF group and the serum total cholesterol concentration was highest in the HPF group (p<0.05). The macrovesicular steatosis was predominant in the HF group, with ballooning hepatocytes and Mallory bodies and in the HPF group predominant microvesicular steatosis was found, without ballooning hepatocytes and Mallory bodies. An increase in TBARS and a decrease in Vitamin E in the HF and HPF groups (p<0.05) was also found. The HF group showed the highest acid oleic deposit in the liver, followed by the HPF group. The SFA hepatic concentration was similar among the groups (p>0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.

Published in	Advances in Biochemistry (Volume 3, Issue 6)
DOI	10.11648/j.ab.20150306.14
Page(s)	86-95
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Hepatic Steatosis, Microvesicular Steatosis, High Fat Diet, High Protein Diet

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APA Style

Daphne Santoro Leonardi-Carvalho, Sergio Zucoloto, Paula Payão Ovidio, Renato Heidor, Thomas Prates Ong, et al. (2015). Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats. Advances in Biochemistry, 3(6), 86-95. https://doi.org/10.11648/j.ab.20150306.14

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ACS Style

Daphne Santoro Leonardi-Carvalho; Sergio Zucoloto; Paula Payão Ovidio; Renato Heidor; Thomas Prates Ong, et al. Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats. Adv. Biochem. 2015, 3(6), 86-95. doi: 10.11648/j.ab.20150306.14

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AMA Style

Daphne Santoro Leonardi-Carvalho, Sergio Zucoloto, Paula Payão Ovidio, Renato Heidor, Thomas Prates Ong, et al. Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats. Adv Biochem. 2015;3(6):86-95. doi: 10.11648/j.ab.20150306.14

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@article{10.11648/j.ab.20150306.14,
  author = {Daphne Santoro Leonardi-Carvalho and Sergio Zucoloto and Paula Payão Ovidio and Renato Heidor and Thomas Prates Ong and Fernando Salvador Moreno and Alceu Afonso Jordao},
  title = {Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats},
  journal = {Advances in Biochemistry},
  volume = {3},
  number = {6},
  pages = {86-95},
  doi = {10.11648/j.ab.20150306.14},
  url = {https://doi.org/10.11648/j.ab.20150306.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20150306.14},
  abstract = {The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.},
 year = {2015}
}

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TY  - JOUR
T1  - Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats
AU  - Daphne Santoro Leonardi-Carvalho
AU  - Sergio Zucoloto
AU  - Paula Payão Ovidio
AU  - Renato Heidor
AU  - Thomas Prates Ong
AU  - Fernando Salvador Moreno
AU  - Alceu Afonso Jordao
Y1  - 2015/12/10
PY  - 2015
N1  - https://doi.org/10.11648/j.ab.20150306.14
DO  - 10.11648/j.ab.20150306.14
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 86
EP  - 95
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20150306.14
AB  - The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.
VL  - 3
IS  - 6
ER  -

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Author Information

Daphne Santoro Leonardi-Carvalho

Department of Pathology, Faculty of Medicine de Ribeir?o Preto, University of S?o Paulo, Avenida Bandeirantes, Ribeir?o Preto-SP, Brasil
Sergio Zucoloto

Department of Pathology, Faculty of Medicine de Ribeir?o Preto, University of S?o Paulo, Avenida Bandeirantes, Ribeir?o Preto-SP, Brasil
Paula Payão Ovidio
Renato Heidor

Department of Food, Faculty of Pharmaceutical Science, University of S?o Paulo, Avenida Prof. Lineu Prestes, S?o Paulo-SP, Brasil
Thomas Prates Ong

Department of Food, Faculty of Pharmaceutical Science, University of S?o Paulo, Avenida Prof. Lineu Prestes, S?o Paulo-SP, Brasil
Fernando Salvador Moreno

Department of Food, Faculty of Pharmaceutical Science, University of S?o Paulo, Avenida Prof. Lineu Prestes, S?o Paulo-SP, Brasil
Alceu Afonso Jordao

Department of Internal Medicine, Faculty of Medicine of Ribeir?o Preto, University of S?o Paulo, Avenida Bandeirantes, Ribeir?o Preto-SP, Brasil

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Daphne Santoro Leonardi-Carvalho, Sergio Zucoloto, Paula Payão Ovidio, Renato Heidor, Thomas Prates Ong, et al. (2015). Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats. Advances in Biochemistry, 3(6), 86-95. https://doi.org/10.11648/j.ab.20150306.14

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ACS Style

Daphne Santoro Leonardi-Carvalho; Sergio Zucoloto; Paula Payão Ovidio; Renato Heidor; Thomas Prates Ong, et al. Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats. Adv. Biochem. 2015, 3(6), 86-95. doi: 10.11648/j.ab.20150306.14

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AMA Style

Daphne Santoro Leonardi-Carvalho, Sergio Zucoloto, Paula Payão Ovidio, Renato Heidor, Thomas Prates Ong, et al. Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats. Adv Biochem. 2015;3(6):86-95. doi: 10.11648/j.ab.20150306.14

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@article{10.11648/j.ab.20150306.14,
  author = {Daphne Santoro Leonardi-Carvalho and Sergio Zucoloto and Paula Payão Ovidio and Renato Heidor and Thomas Prates Ong and Fernando Salvador Moreno and Alceu Afonso Jordao},
  title = {Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats},
  journal = {Advances in Biochemistry},
  volume = {3},
  number = {6},
  pages = {86-95},
  doi = {10.11648/j.ab.20150306.14},
  url = {https://doi.org/10.11648/j.ab.20150306.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20150306.14},
  abstract = {The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.},
 year = {2015}
}

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TY  - JOUR
T1  - Metabolic Differences in the Steatosis Induced by a High-Fat Diet and High-Protein-Fat Diet in Rats
AU  - Daphne Santoro Leonardi-Carvalho
AU  - Sergio Zucoloto
AU  - Paula Payão Ovidio
AU  - Renato Heidor
AU  - Thomas Prates Ong
AU  - Fernando Salvador Moreno
AU  - Alceu Afonso Jordao
Y1  - 2015/12/10
PY  - 2015
N1  - https://doi.org/10.11648/j.ab.20150306.14
DO  - 10.11648/j.ab.20150306.14
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 86
EP  - 95
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20150306.14
AB  - The study aim was to evaluate some effects of a high-fat diet and a high-protein-fat diet in the hepatic steatosis development in rats. Twenty nine Wistar rats were divided at random into three groups: Control: a control diet with soybean oil; HF: a high-fat diet with 50% of lard; HPF: a high-protein-fat diet with about 40% of protein and 50% of lard. The groups were fed with these diets during four weeks. The following parameters were analyzed: hepatic steatosis, serum and hepatic lipid profile, lipid peroxidation, antioxidants concentration and hepatocytes damage. The HF group showed the highest caloric intake per day and the highest weight gain (p0.05), whereas the MUFA concentration was higher in the HF and HPF (p>0.05) than the control group (p<0.05). Therefore, the experimental model used is an efficient model to study hepatic steatosis. The HF and HPF groups had the same behaviors for oxidative stress, serum glucose and hepatic damage in responses to the experimental diets, but these groups showed different esteatosis features.
VL  - 3
IS  - 6
ER  -

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