"MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics

Oleg Gradov; Margaret Gradova

doi:doi:10.11648/j.ab.20150306.11

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"MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics

Oleg Gradov, Margaret Gradova

Published in Advances in Biochemistry (Volume 3, Issue 6)

Received: 26 August 2015 Accepted: 9 October 2015 Published: 30 October 2015

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Abstract

In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or "ion microscopy", live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, etc. We also consider the ways to improve the informativeness of these methods and particularly emphasize the trend at the increasing of the analysis complexity. We propose here the way to improve the efficiency of the cell trapping to the capillary during MS-path-clamp, as well as to provide laser surface ionization using laser trapping and tweezing of cells with the laser beam transmitted through the capillary as a waveguide. It is also possible to combine the above system with the microcolumn separation system or capillary electrophoresis as an optional direction of further development of the complex of analytical techniques emerging from the MS variation of patch-clamp.

Published in	Advances in Biochemistry (Volume 3, Issue 6)
DOI	10.11648/j.ab.20150306.11
Page(s)	66-71
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2015. Published by Science Publishing Group

Keywords

Patch-Clamp, Chanellomics, Scanning Ion Conductance Microscopy, Membrane Receptors, Ion Microscopy, Quantitative Subcellular Secondary Ion Mass Spectrometry, Single-Cell Video-Mass Spectrometry

References

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Oleg Gradov, Margaret Gradova. (2015). "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics. Advances in Biochemistry, 3(6), 66-71. https://doi.org/10.11648/j.ab.20150306.11

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Oleg Gradov; Margaret Gradova. "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics. Adv. Biochem. 2015, 3(6), 66-71. doi: 10.11648/j.ab.20150306.11

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Oleg Gradov, Margaret Gradova. "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics. Adv Biochem. 2015;3(6):66-71. doi: 10.11648/j.ab.20150306.11

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@article{10.11648/j.ab.20150306.11,
  author = {Oleg Gradov and Margaret Gradova},
  title = {"MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics},
  journal = {Advances in Biochemistry},
  volume = {3},
  number = {6},
  pages = {66-71},
  doi = {10.11648/j.ab.20150306.11},
  url = {https://doi.org/10.11648/j.ab.20150306.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20150306.11},
  abstract = {In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or "ion microscopy", live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, etc. We also consider the ways to improve the informativeness of these methods and particularly emphasize the trend at the increasing of the analysis complexity. We propose here the way to improve the efficiency of the cell trapping to the capillary during MS-path-clamp, as well as to provide laser surface ionization using laser trapping and tweezing of cells with the laser beam transmitted through the capillary as a waveguide. It is also possible to combine the above system with the microcolumn separation system or capillary electrophoresis as an optional direction of further development of the complex of analytical techniques emerging from the MS variation of patch-clamp.},
 year = {2015}
}

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TY  - JOUR
T1  - "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics
AU  - Oleg Gradov
AU  - Margaret Gradova
Y1  - 2015/10/30
PY  - 2015
N1  - https://doi.org/10.11648/j.ab.20150306.11
DO  - 10.11648/j.ab.20150306.11
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 66
EP  - 71
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20150306.11
AB  - In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or "ion microscopy", live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, etc. We also consider the ways to improve the informativeness of these methods and particularly emphasize the trend at the increasing of the analysis complexity. We propose here the way to improve the efficiency of the cell trapping to the capillary during MS-path-clamp, as well as to provide laser surface ionization using laser trapping and tweezing of cells with the laser beam transmitted through the capillary as a waveguide. It is also possible to combine the above system with the microcolumn separation system or capillary electrophoresis as an optional direction of further development of the complex of analytical techniques emerging from the MS variation of patch-clamp.
VL  - 3
IS  - 6
ER  -

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Author Information

Oleg Gradov

Talrose Institute for Energy Problems of Chemical Physics, Russian Academy of Sciences, Moscow, Russia
Margaret Gradova

Semenov Institute of Chemical Physics, Russian Academy of Sciences, Moscow, Russia

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Oleg Gradov, Margaret Gradova. (2015). "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics. Advances in Biochemistry, 3(6), 66-71. https://doi.org/10.11648/j.ab.20150306.11

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ACS Style

Oleg Gradov; Margaret Gradova. "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics. Adv. Biochem. 2015, 3(6), 66-71. doi: 10.11648/j.ab.20150306.11

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AMA Style

Oleg Gradov, Margaret Gradova. "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics. Adv Biochem. 2015;3(6):66-71. doi: 10.11648/j.ab.20150306.11

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@article{10.11648/j.ab.20150306.11,
  author = {Oleg Gradov and Margaret Gradova},
  title = {"MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics},
  journal = {Advances in Biochemistry},
  volume = {3},
  number = {6},
  pages = {66-71},
  doi = {10.11648/j.ab.20150306.11},
  url = {https://doi.org/10.11648/j.ab.20150306.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20150306.11},
  abstract = {In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or "ion microscopy", live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, etc. We also consider the ways to improve the informativeness of these methods and particularly emphasize the trend at the increasing of the analysis complexity. We propose here the way to improve the efficiency of the cell trapping to the capillary during MS-path-clamp, as well as to provide laser surface ionization using laser trapping and tweezing of cells with the laser beam transmitted through the capillary as a waveguide. It is also possible to combine the above system with the microcolumn separation system or capillary electrophoresis as an optional direction of further development of the complex of analytical techniques emerging from the MS variation of patch-clamp.},
 year = {2015}
}

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TY  - JOUR
T1  - "MS-Patch-Clamp" or the Possibility of Mass Spectrometry Hybridization with Patch-Clamp Setups for Single Cell Metabolomics and Channelomics
AU  - Oleg Gradov
AU  - Margaret Gradova
Y1  - 2015/10/30
PY  - 2015
N1  - https://doi.org/10.11648/j.ab.20150306.11
DO  - 10.11648/j.ab.20150306.11
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 66
EP  - 71
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20150306.11
AB  - In this projecting work we propose a mass spectrometric patch-clamp equipment with the capillary performing both a local potential registration at the cell membrane and the analyte suction simultaneously. This paper provides a current literature analysis comparing the possibilities of the novel approach proposed with the known methods, such as scanning patch-clamp, scanning ion conductance microscopy, patch clamp based on scanning probe microscopy technology, quantitative subcellular secondary ion mass spectrometry or "ion microscopy", live single-cell mass spectrometry, in situ cell-by-cell imaging, single-cell video-mass spectrometry, etc. We also consider the ways to improve the informativeness of these methods and particularly emphasize the trend at the increasing of the analysis complexity. We propose here the way to improve the efficiency of the cell trapping to the capillary during MS-path-clamp, as well as to provide laser surface ionization using laser trapping and tweezing of cells with the laser beam transmitted through the capillary as a waveguide. It is also possible to combine the above system with the microcolumn separation system or capillary electrophoresis as an optional direction of further development of the complex of analytical techniques emerging from the MS variation of patch-clamp.
VL  - 3
IS  - 6
ER  -

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