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Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province

Received: 4 August 2023     Accepted: 25 August 2023     Published: 8 September 2023
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Abstract

Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.

Published in Advances in Applied Sciences (Volume 8, Issue 3)
DOI 10.11648/j.aas.20230803.16
Page(s) 100-105
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2023. Published by Science Publishing Group

Keywords

Polyscias fruticosa (L.) Harms, Oleanolic Acid, HPLC

References
[1] Vo Van Chi. (2012). Dictionary of Vietnamese Medicinal Plants; Publisher of Medicine: Hanoi, Vietnam, p. 937-938.
[2] Do Tat Loi. (2013). Medicinal Plants and Remedy of Vietnam; Publisher of Hong Duc: Hanoi, Vietnam, p. 828-830.
[3] Asumeng Koffuor G, Boye A, Kyei S, Ofori-Amoah J, Akomanin Asiamah E, Barku A, Acheampong J, Amegashie E, Kumi Awuku A. (2014). Anti-inflammatory and safety assessment of Polyscias fruticosa (L.) Harms leaf extract in ovalbumin-induced asthma. Pharm Biol. 2014; 3 (5); p. 337-342.
[4] M. P. Nguyen. (2020). Impact of roasting to total phenolic, flavonoid and antioxidant activities in root, bark and leaf of Polyscias fruticose. Journal of Pharmaceutical Research International; 32 (2), p. 13–17.
[5] Koffuor, G. A.; Boye, A.; Kyei, S.; Ofori-Amoah, J.; Asiamah, E. A.; Barku, A.; Acheampong, J.; Amegashie, E.; Awuku, A. K. (2016). Anti-asthmatic property and possible mode of activity of an ethanol leaf extract of Polyscias fruticose. Pharm. Biol. 54, 1354–1363.
[6] Khoang, L. T.; Huyen, H. T. T.; Chung, H. V.; Duy, L. X.; Toan, T. Q.; Bich, H. T.; Minh, P. T. H.; Pham, D. T. N.; Hien, T. T. Optimization of Total Saponin Extraction from Polyscias fruticosa Roots Using the Ultrasonic-Assisted Method and Response Surface Methodology. Processes 2022, 10, 2034. https://doi.org/10.3390/pr10102034
[7] Ly, H. T.; Nguyen, T. T. H.; Le, V. M.; Lam, B. T.; Mai, T. T. T.; Dang, T. P. T. (2022). Therapeutic potential of Polyscias fruticosa (L.) Harms leaf extract for parkinson’s disease treatment by Drosophila melanogaster model. Oxid. Med. Cell. Longev. 2022, 5262677.
[8] Peng Xu, Bioa Yu, Chapter One - Chemical synthesis of saponins: An update, Editor (s): David C. Baker, Advances in Carbohydrate Chemistry and Biochemistry, Academic Press, Volume 79, 2021, p. 1-62, https://doi.org/10.1016/bs.accb.2021.11.001.
[9] Ashmawy, N. S., Gad, H. A., Ashour, M. L., El-Ahmady, S. H., & Singab, A. N. B. (2020). The genus Polyscias (Araliaceae): A phytochemical and biological review. Journal of Herbal Medicine, vol 23, 100377. doi: 10.1016/j.hermed.2020.100377.
[10] Huan, V. D.; Yamamura, S.; Ohtani, K.; Kasai, R.; Yamasaki, K.; Nham, N. T.; Chau, H. M. (1998). Oleanane Saponins from Polyscias fruticosa. Phytochemistry. 47, 451–457.
[11] Castellano, José M., Sara Ramos-Romero, and Javier S. Perona. (2022). Oleanolic Acid: Extraction, Characterization and Biological Activity. Nutrients 14, no. 3: 623. https://doi.org/10.3390/nu14030623.
[12] Thai. T. V., Hanh. T. T. H., Dat. N. T.. (2016). Validated High Performance Liquid Chromatography Method for Quantification of a Major Saponin in Polyscias fruticosa. Journal of Multidisciplinary Engineering Science and Technology (JMEST), 3, p. 4880-4882.
[13] Kim. P. T. A., An. L. T. B., Chung. N. T., Truong. N. T., Thu L. T. A, Huan L. V. T. and Loc N. H. (2021). Growth and Oleanolic Acid Accumulation of Polyscias fruticosa Cell Suspension Cultures. Current Pharmaceutical Biotechnology, vol 22, 000-000.
[14] ICH Harmonised tripartite guideline. (2005). Validation of analytical procedures: text and methodology. p. 1-13.
[15] Ludwig Huber (2007), Validation and Qualification in Analytical Laboratories, 2nd edition. p. 144-146.
Cite This Article
  • APA Style

    Le Thi Minh Thu, Pham Hoang Anh. (2023). Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Advances in Applied Sciences, 8(3), 100-105. https://doi.org/10.11648/j.aas.20230803.16

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    ACS Style

    Le Thi Minh Thu; Pham Hoang Anh. Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Adv. Appl. Sci. 2023, 8(3), 100-105. doi: 10.11648/j.aas.20230803.16

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    AMA Style

    Le Thi Minh Thu, Pham Hoang Anh. Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province. Adv Appl Sci. 2023;8(3):100-105. doi: 10.11648/j.aas.20230803.16

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  • @article{10.11648/j.aas.20230803.16,
      author = {Le Thi Minh Thu and Pham Hoang Anh},
      title = {Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province},
      journal = {Advances in Applied Sciences},
      volume = {8},
      number = {3},
      pages = {100-105},
      doi = {10.11648/j.aas.20230803.16},
      url = {https://doi.org/10.11648/j.aas.20230803.16},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20230803.16},
      abstract = {Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.},
     year = {2023}
    }
    

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    T1  - Determination of Oleanolic Acid Content in Radix Polysciacis in Tra Vinh Province
    AU  - Le Thi Minh Thu
    AU  - Pham Hoang Anh
    Y1  - 2023/09/08
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    T2  - Advances in Applied Sciences
    JF  - Advances in Applied Sciences
    JO  - Advances in Applied Sciences
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    EP  - 105
    PB  - Science Publishing Group
    SN  - 2575-1514
    UR  - https://doi.org/10.11648/j.aas.20230803.16
    AB  - Polyscias fruticosa (L.) Harms is a traditional medicine, which is planted widely in Tra Vinh province of Vietnam. It is used for nourishing the body, curing asthenia, emaciation, fatigue, poor digestion, poor metabolism, enhance working capacity and brain activity. Oleanolic acid (OA), a marker, is a sapogenin in radix polysciacis. Quantification of OA by high performance liquid chromatography (HPLC) makes an important contribution to the quality assessment of the medicinal plant of Polyscias fruticosa (L.) Harms. In order to develop and apply a method to evaluate the content OA in radix polysciacis, planted in Tra Vinh province, many conditions for extracting OA from radix polysciacis were investigated; many HPLC testing conditions were performed find a simple, accurate and fast analytical procedure for the quantification of OA in extract solution of radix polysciacis. As a result, the optimum extraction procedure of OA from the roots of Polyscias fruticosa (L.) Harms was found out; a simple, accurate and fast analytical procedure for the quantification of OA in radix polysciacis’s extract solution was developed, evaluated and applied to assets the OA content of 05 samples of radix polysciacis collected in different regions of Tra Vinh province. This developed method can control the quality of medicinal materials of Polyscias fruticosa (L.) Harms and its preparation effectively.
    VL  - 8
    IS  - 3
    ER  - 

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Author Information
  • Faculty of Pharmacy, Nguyen Tat Thanh University, Ho Chi Minh City, Viet Nam

  • Faculty of Pharmacy, Pham Ngoc Thanh University of Medicine, Ho Chi Minh City, Viet Nam

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