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Ethanol Production by Alcohol Tolerant Yeasts Using Different Carbohydrate Sources

Received: 13 October 2016     Accepted: 27 October 2016     Published: 16 October 2017
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Abstract

This study aimed at comparing the ability of two indigenous yeast species; Pichia kudriavzevii strains GY1 and L9 with a strain of Saccharomyces cerevisiae, to consume sugars (fructose, galactose, glucose, lactose, sucrose and molasses) and to convert them into ethanol during fermentation. Yeast extract (6g/L), peptone (10g/L), malt extract (6g/L) broth was supplemented with different concentrations (5g/L, 10g/L, 20g/L, 30g/L) of fructose, galactose, glucose, lactose and sucrose respectively. Sugar utilization post incubation for 96 hours at 120 rpm, 30 degree Celsius (°C) was measured using a refractometer. The alcoholic yield using molasses for Pichia kudriavzevii strain GY1 10±0.2 (mg/ml) was significantly higher than that of Pichia kudriavzevii strain L9 (4±0.2 mg/ml) and Saccharomyces cerevisiae strain T (5±0.2 mg/ml) at 96 hours. Strains that produced highest concentration ethanol was Pichia kudriavzevii strain L9 in 3.0% (v/v) galactose and fructose respectively, which measured at 7.1±0.48 (mg/ml) and 12.2±0.64 (mg/ml). All studied isolates produced the same amount of ethanol 9.1±0.52 (mg/ml). The use of highly adaptable non Saccharomyces yeast species to a variety of sugars in the pursuit of enhanced ethanol production creates a unique prospective for large scale industrial applications.

Published in Advances in Applied Sciences (Volume 2, Issue 5)
DOI 10.11648/j.aas.20170205.13
Page(s) 69-74
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2017. Published by Science Publishing Group

Keywords

Alcoholic Fermentation, Pichia kudriavzevii, Fructose, Glucose, Galactose, Lactose, Sucrose

References
[1] Khaw, TS., Katakura, Y., Ninomiya, K., Moukamnerd, C., Kondo, A., Ueda, M., and Shioya, S., (2007). Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation. Journal of Bioscience and Bioengineering, 103: 95-97.
[2] Hu, Z., and Wen, Z., (2008). Enhancing enzymatic digestibility of switchgrass by microwave-assisted alkali pretreatment. Journal of Biochemical Engineering, 38: 369-378.
[3] Moreira, JR., (2000). Sugar cane for energy recent results and progress in Brazil. Energy and Sustainable Development, 17: 43-54.
[4] Ragauskas, AJ., Williams, CK., Davison, BH., Britovsek, G., Cairney, J., Eckert, CA., Frederick, WJ., Hallett, JP., Leak, DJ., Liotta, CL., Mielenz, JR., Murphy, R., Templer, R., and Tschaplinski, T., (2006). The path forward for biofuels and biomaterials. Science, 311: 484-489.
[5] Matsakas, L., and Christakopoulos, P., (2015). Ethanol Production from Enzymatically Treated Dried Food Waste Using Enzymes Produced On-Site. Sustainability, 7: 1446-1458.
[6] Dake, MS., Amarapurkar, SV., Salunkhe, ML., and Kamble, SR., (2010). Production of Alcohol by Saccharomyces sp. using Natural Carbohydrate Sources. Advanced Biotechnology, 10: 37-41.
[7] Dhaliwal, SS., Oberoi, HS., Sandhu, SK., Nanda, D., Kumar, D., and Uppal, SK., (2011). Enhanced ethanol production from sugarcane juice by galactose adaptation of a newly isolated thermotolerant strain of Pichia kudriavzevii. Bioresource Technology, 102: 5968-5975.
[8] Izmirlioglu, G., and Demirci, A., (2012). Ethanol Production from Waste Potato Mash by Using Saccharomyces cerevisiae. Applied Sciences, 2: 738-753.
[9] Balat, M., Balat, H., and Oz, C., (2008). Progress in bioethanol processing. Progress in Energy and Combustion, 34: 551-573.
[10] Abdel-Banat, BMA., Hoshida, H., Ano, A., Nonklang, S., and Akada, R., (2009). High temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast. Applied Microbiology & Biotechnology, 85: 861-867.
[11] Porro, D., and Branduardi, P., (2009). Yeast cell factory: fishing for the best one or engineering it? Microbial Cell Factories, 8: 51.
[12] Lane, MM., and Morrissey, JP., (2010). Kluyveromyces marxianus: A yeast emerging from its sister’s shadow. Fungal Biology Reviews, 24: 7-26.
[13] Gidado, RSM., Etim, VA., Nweke, O., Iloh, AC., Isu, RN., and Solomon, BO., (2016). Isolation and Identification of Local Ethanol Tolerant Yeast Populating Distillation and Milling Sites in Nigeria. American Journal of BioScience, 4: 58-63.
[14] Fonseca, GG., Gombert, AK., Heinzle, E., and Wittmann, C., (2007). Physiology of the yeast Kluyveromyces marxianus during batch and chemostat cultures with glucose as the sole carbon source. FEMS Yeast Research, 7: 422-435.
[15] Signori, L., Passolunghi, S., Ruohonen, L., Porro, D., and Branduardi, P., (2014). Effect of oxygenation and temperature on glucose-xylose fermentation in Kluyveromyces marxianus CBS712 strain. Microbial Cell Factories, 13: 51-64.
[16] Rodrussamee, N., Lertwattanasakul, N., Hirata, K., Suprayogi-Limtong, S., Kosaka, T., and Yamada, M., (2011). Growth and ethanol fermentation ability on hexose and pentose sugars and glucose effect under various conditions in thermotolerant yeast Kluyveromyces marxianus. Applied Microbiology and Biotechnology, 90: 1573-1586.
[17] Tronchoni, J., Gamero, A., Arroyo-Lopez, F. N., Barrio, E., and Querol, A., (2009). Differences in the glucose and fructose consumption profiles in diverse Saccharomyces wine species and their hybrids during grapes juice fermentation. Industrial Journal of Food Microbiology, 134: 237-243.
[18] Berthels, N. J., Otero, R. R. C., Bauer, F. F., Thevelein, J. M., and Pretorius, I. S., (2004). Discrepancy in glucose and fructose utilization during fermentation by Saccharomyces cerevisiae wine yeast. FEMS Yeast Research, 4: 683-689.
[19] Berthels, N. J., Otero, R. R. C., Bauer, F. F., and Pretorius, I. S. (2008). Correlation between glucose/fructose discrepancy and hexokinase kinetic properties in different Saccharomyces cerevisiae wine yeast strains. Applied Microbiology and Biotechnology, 77: 1083-1091.
[20] Wu, X., Staggenborg, S., Propheter, J. L., Rooney, W. L., Yu, J., and Wang, D., (2010). Feature of sweet sorghum juice and their performance in ethanol fermentation. Industrial Crops and Products, 31: 164-170.
[21] Parrondo, J., García, L. A., and Díaz, M., (2007). Nutrient balance and metabolic analysis in a Kluyveromyces marxianus fermentation with lactose-added whey. Brazilian Journal of Chemical Engineering, 26: 445-456.
[22] Rodrussamee, N., Lertwattanasakul, N., Hirata, K., Suprayogi, L. S., Kosaka, T., and Yamada, M., (2011). Growth and ethanol fermentation ability on hexose and pentose sugars and glucose effect under various conditions in thermotolerant yeast Kluyveromyces marxianus. Applied Microbiology and Biotechnology, 90: 1573-1586.
Cite This Article
  • APA Style

    Gidado Rose Suniso Maxwell, Olatiilu Olukemi Anna, Etuk-Udo Godwin Akpan, Isu Rosemary Nennaya, Solomon Bamidele Ogbe. (2017). Ethanol Production by Alcohol Tolerant Yeasts Using Different Carbohydrate Sources. Advances in Applied Sciences, 2(5), 69-74. https://doi.org/10.11648/j.aas.20170205.13

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    ACS Style

    Gidado Rose Suniso Maxwell; Olatiilu Olukemi Anna; Etuk-Udo Godwin Akpan; Isu Rosemary Nennaya; Solomon Bamidele Ogbe. Ethanol Production by Alcohol Tolerant Yeasts Using Different Carbohydrate Sources. Adv. Appl. Sci. 2017, 2(5), 69-74. doi: 10.11648/j.aas.20170205.13

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    AMA Style

    Gidado Rose Suniso Maxwell, Olatiilu Olukemi Anna, Etuk-Udo Godwin Akpan, Isu Rosemary Nennaya, Solomon Bamidele Ogbe. Ethanol Production by Alcohol Tolerant Yeasts Using Different Carbohydrate Sources. Adv Appl Sci. 2017;2(5):69-74. doi: 10.11648/j.aas.20170205.13

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  • @article{10.11648/j.aas.20170205.13,
      author = {Gidado Rose Suniso Maxwell and Olatiilu Olukemi Anna and Etuk-Udo Godwin Akpan and Isu Rosemary Nennaya and Solomon Bamidele Ogbe},
      title = {Ethanol Production by Alcohol Tolerant Yeasts Using Different Carbohydrate Sources},
      journal = {Advances in Applied Sciences},
      volume = {2},
      number = {5},
      pages = {69-74},
      doi = {10.11648/j.aas.20170205.13},
      url = {https://doi.org/10.11648/j.aas.20170205.13},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.aas.20170205.13},
      abstract = {This study aimed at comparing the ability of two indigenous yeast species; Pichia kudriavzevii strains GY1 and L9 with a strain of Saccharomyces cerevisiae, to consume sugars (fructose, galactose, glucose, lactose, sucrose and molasses) and to convert them into ethanol during fermentation. Yeast extract (6g/L), peptone (10g/L), malt extract (6g/L) broth was supplemented with different concentrations (5g/L, 10g/L, 20g/L, 30g/L) of fructose, galactose, glucose, lactose and sucrose respectively. Sugar utilization post incubation for 96 hours at 120 rpm, 30 degree Celsius (°C) was measured using a refractometer. The alcoholic yield using molasses for Pichia kudriavzevii strain GY1 10±0.2 (mg/ml) was significantly higher than that of Pichia kudriavzevii strain L9 (4±0.2 mg/ml) and Saccharomyces cerevisiae strain T (5±0.2 mg/ml) at 96 hours. Strains that produced highest concentration ethanol was Pichia kudriavzevii strain L9 in 3.0% (v/v) galactose and fructose respectively, which measured at 7.1±0.48 (mg/ml) and 12.2±0.64 (mg/ml). All studied isolates produced the same amount of ethanol 9.1±0.52 (mg/ml). The use of highly adaptable non Saccharomyces yeast species to a variety of sugars in the pursuit of enhanced ethanol production creates a unique prospective for large scale industrial applications.},
     year = {2017}
    }
    

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  • TY  - JOUR
    T1  - Ethanol Production by Alcohol Tolerant Yeasts Using Different Carbohydrate Sources
    AU  - Gidado Rose Suniso Maxwell
    AU  - Olatiilu Olukemi Anna
    AU  - Etuk-Udo Godwin Akpan
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    JF  - Advances in Applied Sciences
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    SN  - 2575-1514
    UR  - https://doi.org/10.11648/j.aas.20170205.13
    AB  - This study aimed at comparing the ability of two indigenous yeast species; Pichia kudriavzevii strains GY1 and L9 with a strain of Saccharomyces cerevisiae, to consume sugars (fructose, galactose, glucose, lactose, sucrose and molasses) and to convert them into ethanol during fermentation. Yeast extract (6g/L), peptone (10g/L), malt extract (6g/L) broth was supplemented with different concentrations (5g/L, 10g/L, 20g/L, 30g/L) of fructose, galactose, glucose, lactose and sucrose respectively. Sugar utilization post incubation for 96 hours at 120 rpm, 30 degree Celsius (°C) was measured using a refractometer. The alcoholic yield using molasses for Pichia kudriavzevii strain GY1 10±0.2 (mg/ml) was significantly higher than that of Pichia kudriavzevii strain L9 (4±0.2 mg/ml) and Saccharomyces cerevisiae strain T (5±0.2 mg/ml) at 96 hours. Strains that produced highest concentration ethanol was Pichia kudriavzevii strain L9 in 3.0% (v/v) galactose and fructose respectively, which measured at 7.1±0.48 (mg/ml) and 12.2±0.64 (mg/ml). All studied isolates produced the same amount of ethanol 9.1±0.52 (mg/ml). The use of highly adaptable non Saccharomyces yeast species to a variety of sugars in the pursuit of enhanced ethanol production creates a unique prospective for large scale industrial applications.
    VL  - 2
    IS  - 5
    ER  - 

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Author Information
  • Agricultural Biotechnology Department, National Biotechnology Development Agency (NABDA), Abuja, Nigeria

  • Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria

  • Biotechnology Advanced Research Center, Sheda Science and Technology Complex (SHESTCO), Abuja, Nigeria

  • Department of Biological Sciences, University of Abuja, Abuja, Nigeria

  • Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Nigeria

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