Excitatory synapses play a fundamental role in neural communication, and their proper maturation and plasticity are crucial for normal brain function. This study focuses on SynDIG1, a key protein that has emerged as a dominant regulator in this process, we want to investigate how SynDIG1 molecule to work during synapse maturation and plasticity. We used plasmid transfection and viral infection to change the expression of SynDIG1, we detected the expression content of molecules by immunocytochemistry and western blot, and analysize the interaction between SynDIG1 and GluA1 by immunopecipitation. The finding shows that SynDIG1 is required molecular to synapses mature and neurons development, it also play a key role in synaptic plasticity. SynDIG1 influence and control GluA1 expression in positive correlation, and these two molecules interact with each other to form a cluster. Based on these results, we draw the conclusion: SynDIG1 regulate the maturation, development and plasticity by interaction with GluA1, which influences the formation and organization of synapses, ensuring the correct establishment of neural circuits. It modulates the trafficking and surface expression of GluA1, thereby affecting the strength of synaptic transmission and the ability of synapses.

SynDIG1, GluA1, Excitatory Synapse, Maturation, Plasticity